| 光谱学与光谱分析 |
|
|
|
|
|
| Research on Detecting Concentration of Serum Protein Based on Resonance Rayleigh Scattering |
| WANG Gao, FENG Qiao-ling, XUE Zhong-jin, LI Yang-jun, ZHOU Han-chang |
1. Key Laboratory of Instrumentation Science and Dynamic Measurement, North University of China, Taiyuan 030051, China
2. National Key Laboratory for Electronic Measurement Technology, North University of China, Taiyuan 030051, China |
|
|
|
|
Abstract The resonance Rayleigh scattering spectral detection system was designed based on the 2,9,16,23-tetracarboxylate-phthalocyanine zinc and protein system. In the system, excitation light source is 405 nm wide band gap semiconductor lasers, and monochromator is 475 nm narrow-band band-pass filter, and the detector is low-noise and high-gain photoelectric amplifier based on blue-ray enhanced photodiode. Experiment shows that, the solution’s strong absorption wavelength is near 420 nm. Under the action of incentive light, resonance Rayleigh scattering is generated at the resonant wavelength, and the scattering intensity is proportional to the protein content. The system uses 2,9,16,23-tetracarboxylate as the spectrum probe to determine the concentration of serum proteins by resonance Rayleigh scattering method. Its linear detection range is 10 ~ 50 mg·mL-1, and its detection limit is 0.001 mg·mL-1. The newly developed device for detecting concentration of the serum protein has the advantages of small size, low cost, low power consumption, and being easy to use.
|
|
Received: 2012-08-01
Accepted: 2012-10-30
|
|
|
|
Corresponding Authors:
WANG Gao
E-mail: gxx@nuc.edu.cn
|
|
[1] Axel Warsinke. Anal. Bioanal. Chem., 2009, 393: 1393.
[2] David J Perry, David A Fitzmaurice, Steve Kitchenl. British Journal of Haematology, 2010，150: 501.
[3] Stolz H. Physica Status Solidi B-Basic Research, 2002, 1(234): 107.
[4] Mohd Ayaz. Journal of Fluorescence, 2011, 4(21): 1357.
[5] LIU Shao-pu, LIU Zhong-fang(刘绍璞，刘忠芳). Acta Chimica Sinica(化学学报)，1995，53(12): 1178.
[6] LIU Shao-pu, HU Xiao-li, LUO Hong-qun(刘绍璞，胡小莉，罗红群). Science China(中国科学)，2002，1(32): 18.
[7] WANG Mei, HAN Quan, YANG Xiao-hui(王 媚，韩 权，杨晓慧). Chinese Journal of Analysis Laboratory(分析试验室)，2010，11(29): 65.
[8] Liu Jinfang, Li Nianbing, Luo Hongqun. Spectrochimica Acta, 2011, (79): 631.
[9] LUO Jia-gang, LIU Zhong-fang, LIU Shao-pu(罗家刚，刘忠芳，刘绍璞). Acta Chimica Sinica(化学学报)，2008，66(23): 2604.
[10] Meyer T, Braun H, Schwarz U T. Optics Express, 2008，16(10): 6833.
[11] Ali Mohd Sajid. Journal of Colloid and Interface Science, 2010, 2(352): 436.
[12] PANG Xiang-dong, ZHENG Xiao-feng, DENG Qin-xin(庞向东, 郑晓锋, 邓钦心). Journal of Analytical Science(分析科学学报)，2011, 2: 84.
[13] ZHANG Min, WU Li-min, ZHOU Shang(张 敏, 吴利敏, 周 尚). Journal of Instrumental Analysis(分析测试学报)，2012, 6: 8.
[14] HAN Quan, WANG Mei, TIAN Li(韩 权, 王 媚, 田 丽). Chinese Journal of Spectroscopy Laboratory(光谱实验室)，2010, 6: 44. |
| [1] |
LIANG Ya-quan1, PENG Wu-di1, LIU Qi1, LIU Qiang2, CHEN Li1, CHEN Zhi-li1*. Analysis of Acetonitrile Pool Fire Combustion Field and Quantitative
Inversion Study of Its Characteristic Product Concentrations[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3690-3699. |
| [2] |
BAI Bing1, 2, 3, CHEN Guo-zhu2, 3, YANG Wen-bin2, 3, CHE Qing-feng2, 3, WANG Lin-sen2, 3, SUN Wei-min1*, CHEN Shuang1, 2, 3*. The Study on Precise and Quantitative Measurement of Flame OHConcentration by CRDS-CARS-PLIF Techniques[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3955-3962. |
| [3] |
DUAN Ming-xuan1, LI Shi-chun1, 2*, LIU Jia-hui1, WANG Yi1, XIN Wen-hui1, 2, HUA Deng-xin1, 2*, GAO Fei1, 2. Detection of Benzene Concentration by Mid-Infrared Differential
Absorption Lidar[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(11): 3351-3359. |
| [4] |
SHEN Ying, WU Pan, HUANG Feng*, GUO Cui-xia. Identification of Species and Concentration Measurement of Microalgae Based on Hyperspectral Imaging[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(11): 3629-3636. |
| [5] |
KANG Ying1, ZHUO Kun1, LIAO Yu-kun1, MU Bing1, QIN Ping2, LI Qian1, LUAN Xiao-ning1*. Quantitative Determination of Alcohol Concentration in Liquor Based on Polarized Raman Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(09): 2768-2774. |
| [6] |
CHANG Zhen1, ZHONG Ming-yu2*, SU Jing-ming1, 2, SI Fu-qi1, WANG Yu3, ZHOU Hai-jin1, DOU Ke1, ZHANG Quan1. Study on the Reconstructing the NO2 Gas Distribution in a Vertical Plane Using MAX-DOAS[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(08): 2413-2418. |
| [7] |
PU Gui-juan1, 2, CHENG Si-yang3*, LI Song-kui4, LÜ Jin-guang2, CHEN Hua5, MA Jian-zhong3. Spectral Inversion and Variation Characteristics of Tropospheric NO2
Column Density in Lhasa, Tibet[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(06): 1725-1730. |
| [8] |
ZHANG Rong1, 2, DUAN Ning1, 3, JIANG Lin-hua1, 3*, XU Fu-yuan3, JIN Wei3, LI Jian-hui1. Study on Optical Path Optimization for Direct Determination of
Spectrophotometry of High Concentration Hexavalent Chromium
Solution by Ultraviolet Visible Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(06): 1829-1837. |
| [9] |
ZHANG Li-fang1, YANG Yan-xia1, ZHAO Guan-jia1, MA Su-xia1, GUO Xue-mao2. Comparison of Numerical Iterative Algorithms for Two-Dimensional Absorption Spectral Reconstruction[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(05): 1367-1375. |
| [10] |
XU Qi-lei, GUO Lu-yu, DU Kang, SHAN Bao-ming, ZHANG Fang-kun*. A Hybrid Shrinkage Strategy Based on Variable Stable Weighted for Solution Concentration Measurement in Crystallization Via ATR-FTIR Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(05): 1413-1418. |
| [11] |
PENG Wei, YANG Sheng-wei, HE Tian-bo, YU Ben-li, LI Jin-song, CHENG Zhen-biao, ZHOU Sheng*, JIANG Tong-tong*. Detection of Water Vapor Concentration in Sealed Medicine Bottles Based on Digital Quadrature Phase-Locked Demodulation Algorithm and TDLAS
Technology[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(03): 698-704. |
| [12] |
ZHANG Qi-jin1, 2, GUO Ying-ying1, 2, LI Su-wen1, 2*, MOU Fu-sheng1, 2*. Study on the Methods of Collecting Atmospheric Pollution Gases in
Huaibei Region by Mobile Mini-DOAS[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(03): 984-989. |
| [13] |
LOU Deng-cheng, RAO Wei*, SONG Jun-ling, WANG Kai, JIANG Ya-jing, GUO Jian-yu. Research of Carbon Monoxide Concentration Measurement in Combustion Field by Off-Axis Integrated Cavity Output Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(12): 3678-3684. |
| [14] |
HAN Jia-qing1, ZHOU Gui-xia1*, HU Jun1*, CHENG Jie-hong2, CHEN Zheng-guang2, ZHAO Sheng-xue1, LIU Yi-ling1. Near-Infrared Spectroscopy Detection of Pollution Concentration of Agricultural Machinery Lubricating Oil Based on Improved Random
Frog Algorithm[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(11): 3482-3488. |
| [15] |
SUN You-rui1, GUO Mei1, LIU Gui-shan1*, FAN Nai-yun1*, ZHANG Hao-nan2, LI Yue1, PU Fang-ning2, YANG Shi-hu1, WANG Hao2. Fusion of Visible Near-Infrared (VNIR) Hyperspectral Imaging and Texture Feature for Prediction of Total Phenolics Content in
Tan Mutton[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(11): 3631-3636. |
|
|
|
|
