光谱学与光谱分析 |
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Study on Method of Time-Resolved Fluorescence Spectra for Rare Fluorescence Chelate |
LI Yun-hui1, DI Yan-qing1, YANG Xiu-yun1, PAN Li-hua2*, LI Jun-ling2, WANG Yun-lei2, LI Hai-yan1, CHANG Yu2, LIU Wen-yu1 |
1. College of Chemistry and Environmental Engineering, Changchun University of Science and Technology, Changchun 130022, China 2. Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, National Analytical Research Center of Electrochemistry and Spectroscopy, Changchun 130022, China |
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Abstract In the present paper, the technology of time-resolved fluorescence spectra was investigated in order to improve the sensitivity of the solid-phase time-resolved fluorescence immunoassay. The fluorescence properties,fluorescence intensity and detection method of the novel complexes 4,4′-dibromo-6,6′-bis(N,N-bis(ethoxycarbonylmethyl)amino methyl)-2,2′-bipyridine were investigated through self-developed solid-phase time-resolved fluorescence immunoassay instrument. At the same time, the Eu-TTA-phen and Eu-β-NTA-TOPO series of Eu(Ⅲ) compounds were detected.
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Received: 2009-10-10
Accepted: 2010-01-20
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Corresponding Authors:
PAN Li-hua
E-mail: lihuap@ciac.jl.cn
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