| 光谱学与光谱分析 |
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| Spectrometric Investigation of the Antioxidant Activity of a Novel Synthetic Selenadiazole Derivative SPO Against DPPH and ABTS Free Radicals |
| ZHANG Yi-bo1, ZHENG Wen-jie2, HUANG Zhi1, YANG Fang2, LIU Jie2, CHEN Tian-feng2,3* |
1. Department of Biology, Jinan University, Guangzhou 510632, China
2. Department of Chemistry, Jinan University, Guangzhou 510632, China
3. Department of Biology, The Chinese University of Hong Kong, Hong Kong, China |
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Abstract The antioxidant activity of a novel synthetic selenadiazole derivative SPO against DPPH and ABTS free radicals was evaluated using spectrometric methods. The results show that the detection wavelength and stable time for DPPH system were 515 nm and 30 min respectively, while those for ABTS system were 734 nm and 6 min, respectively. SPO could effectively and rapidly inhibited the formation of ABTS and DPPH free radicals in a dose- and time-dependent manner, indicating the potent antioxidant activity of SPO under both hydrophilic and hydrophobic conditions. In the optimized systems, the IC50 values of SPO were 85.2 μmol·L-1 (DPPH assay) and 36.5 μmol·L-1 (ABTS assay), respectively, which were comparable with the standard antioxidant Trolox, and significantly better than the positive controls BHA and BHT. Taken together, our results suggest the potential applications of selenadiazole derivatives as antioxidative agents.
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Received: 2009-08-09
Accepted: 2009-11-12
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Corresponding Authors:
CHEN Tian-feng
E-mail: ctf12343@yahoo.com.cn, tchentf@jnu.edu.cn
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[1] Surh Y J. Nat. Rev. Cancer, 2003, 3：768.
[2] Rayman M P. Lancet, 2000, 356：233.
[3] El-Bayoumy K, Sinha R. Mutation Res., 2004, 551：181.
[4] Tapiero H, Townsend D M, Tew K D. Biomed. Pharmacother., 2003, 57：134.
[5] Abdulah R, Miyazaki K, Nakazawa M, et al. J. Trace. Elem. Med. Biol., 2005, 19：141.
[6] ZHENG Wen-jie, ZENG Xin-hua, YANG Fang, et al(郑文杰，曾鑫华，杨 芳, 等). Journal of Analytical Science(分析科学学报), 2003, 19(1)：36.
[7] ZOU Jia-hao, ZHENG Wen-jie, YANG Fang, et al(邹家浩，郑文杰，杨 芳，等). Journal of Analytical Science(分析科学学报), 2005, 21(4)：381.
[8] ZHANG Jun-ling, ZOU Jia-hao, ZHENG Wen-jie, et al(张俊苓，邹家浩，郑文杰, 等). Chemical Research and Application(化学研究与应用), 2004, 16(4)：561.
[9] Zhang J L, Zheng W J, Zou J H, et al. Chem. J. Int., 2004，6：97.
[10] Chen T F, Wong Y S, Zheng W J, et al. Chemico-Biological Interact., 2009, 180：54.
[11] Chen T F, Zheng W J, Wong Y S, et al. Biomed. Pharmacother., 2008, 62：77.
[12] Shyu Y S, Lin J T, Chang Y T, et al. Food Chem., 2009, 115：515.
[13] GUO Xue-feng, YUE Yong-de, TANG Feng, et al(郭雪峰, 岳永德, 汤 锋, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2008, 28(7)：1578.
[14] Sharma O P, Bhat T K. Food Chem., 2009, 113：1202.
[15] Marghitas L A, Oltica G S, Daniel S D, et al. Food Chem., 2009, 115：878.
[16] Chen T F, Wong Y S. J. Agric. Food Chem., 2008, 56：4352.
[17] Miller N J, Sampson J, Candeias L P, et al. FEBS Lett.，1996, 384：240.
[18] De Beer D, Joubert E, Gelderblom W C A, et al. J. Agric. Food Chem., 2003, 51(4)：902. |
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