光谱学与光谱分析 |
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Difference Expressed Protein Study on Unstable Angina Blood-Stasis Syndrome by Fluorescence Labelling Method |
ZHAO Hui-hui1,HOU Na2, WANG Wei2* |
1. Beijing University of Chinese Medicine,Beijing 100029,China 2. Beijing Hospital of Traditional Chinese Medicine, Beijing 100010 China |
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Abstract Fluorescence labelling method was used seeking differentially expressed plasma proteins of unstable angina blood-stasis syndrome. Three fluorescence labelling dyes (Cy2, Cy3 and Cy5) were used on two classes of species (plasma of 12 patients with unstable angina blood-stasis syndrome and 12 healthy volunteers) Two dimensional gel electrophoresis was carried out, and three different fluorescence scans were performed. By analyzing the graphs and mass spectrometry, the differentially expressed plasma proteins of unstable angina were obtained. Blood-stasis syndrome. Results show that fibrinogen β chain, fibrinogen γ chain, α1-antitrypsin, haptoglobin β chain and haptoglobin α2 chain were significantly highly expressed in the plasma of unstable angina blood-stasis syndrome patient,while ApoA-Ⅳ, ApoA-Ⅰ and transthyretin were decreased in the plasma of unstable angina blood-stasis syndrome patient. These identified proteins could be divided into two categories according to their functions: (1) Acute phase reactive protein; (2) Apolipoprotein. In conclusion, fibrinogen β chain, fibrinogen γ chain, α1-antitrypsin, transthyretinc, haptoglobin β chain, ApoA-Ⅳ, ApoA-Ⅰ and haptoglobin α2 chain are differentially expressed in the patients with unstable angina blood-stasis syndrome and control group, some differentially expressed proteins were correlated with inflammatory reaction or lipid metabolic disorder, and these proteins could provide clues to the study and discovery of new protein targets for antianginal drugs. The fluorescence labelling method is suitable for discovery of differentially expressed proteins.
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Received: 2008-03-06
Accepted: 2008-06-08
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Corresponding Authors:
WANG Wei
E-mail: wangwei26960@126.com
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[1] ZHAO Hui-hui,WANG Wei,WANG Shuo-ren,et al(赵慧辉, 王 伟, 王硕仁, 等). Chinese Archives of Traditional Chinese Medicine(中华中医药学刊), 2008, 26(4): 724. [2] Anderson N L, Anderson N G. Mol. Cell Proteomics, 2002, 1(11): 845. [3] Van den Bergh G, Clerens S, Vandesande F, et al. Electrophoresis, 2003, 24(9): 1471. [4] Huang H L, Stasyk T, Morandell S, et al. Electrophoresis, 2006, 27(8): 1641. [5] ZHAO Hui-hui, WANG Wei(赵慧辉, 王 伟). Acta Chimca Sinica(化学学报), 2009, 67(2): 167. [6] Dobryszycka W. Eur. J. Clin. Chem. Clin. Biochem., 1997, 35(9): 647. [7] LIU Yang-qing, YUAN Rong, ZHAO Hui-hui, et al(刘养清,苑 戎, 赵慧辉, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2002,22(5): 779. [8] Heliovaara M K, Teppo A M, Karonen S L, et al. Diabetes Obesity and Metabolism, 2005, 7(6): 729. [9] Daemen Marc A R C, Heemskerk Vincent H, Veer Cornelis van ’t, et al. Circulation, 2000, 102: 1420. [10] James J S, Halit S, Geoffrey H T, et al. Circulation, 2000, 102: 1634. [11] Dvorin Elena, Corder Nancy L, Benson Douglas M, et al. J. Biological Chemistry, 1986, 261(33): 15714. [12] Cohen Robert D, Castellani Lawrence W, Qiao Jian-Hua, et al. J. Clin. Investigation, 1997, 99(8): 1906. [13] Fournier T,Medjoubi N N,Porquet D. Biochim. Biophys. Acta, 2000, 1482:157. |
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