Study of Synthesis and Spectral Property of Europium Cryptate
SUN Yan-wen1, CHANG Yu2, JIN Yu-fen1, XIE Wen-bing2, CHANG Jing1, YU Ting1*, PAN Li-hua2
1. The Second Hospital of Jilin University, Changchun 130041, China
2. Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China
Abstract：Synthesis of rare-earth chelate is a key part of homogeneous time-resolved fluorescence immunoassay. To synthesize optimal rare-earth chelate, we select diethyl 2,6-dibromomethyl-3,5-pyridinecarboxylate as raw material and optimally synthesize Li+⊂2,6-{N,N’,N,N’-[bis(2,2’-bipyridine-6,6’-dimethyl)] bis(aminomethyl)}-pyridine-3,5-diethyl diester，and then the spectral difference of europium cryptate synthesized by the different reactive systems of acetonitrile and methyl alcohol are discussed. Studies show the excitation spectra(the maximum excitation wavelength is 310 nm), emission spectra(the maximum emission wavelength is 616 nm) and quantum yield of europium cryptate synthesized by the different reactive systems are consistent. Fluorescence intensity are linear with the concentration of europium in the range of 10-8～10-5 mol·L-1 (R2=0.993 73, 0.986 65), respectively. But the fluorescence intensity between them (c=2.5×10-5 mol·L-1) are slightly different. Their fluorescence lifetime are 825 and 830 μs, respectively. Therefore, europium cryptate synthesized has big Stoke’s shift, high fluorescence intensity, long fluorescence lifetime and so on. Pyridine-2,2-bipyridine in europium cryptate can protect europium from interference. It is an ideal europium cryptate used for the labeling of biomolecules such as protein, nucleic acid. This study not only expands the synthesis of new rare earth chelate，but also lays the foundation for the establishment of homogeneous time-resolved fluorescence immunoassay.
孙延文，常 宇，金玉芬，谢文兵，常 静，于 庭，潘利华. 铕螯合物的制备及光谱性质研究[J]. 光谱学与光谱分析, 2018, 38(07): 2189-2193.
SUN Yan-wen, CHANG Yu, JIN Yu-fen, XIE Wen-bing, CHANG Jing, YU Ting, PAN Li-hua. Study of Synthesis and Spectral Property of Europium Cryptate. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(07): 2189-2193.
[1] Philippe，Nakache，E. Omri，et al. U. S. Patent，WO/2013/042120，2013.
[2] Blanc E，Wagner P，Plaisier F，et al. Analytical Biochemistry，2015，484: 105.
[3] O’Malley W I，Abdelkader E H，Aulsebrook M L，et al. Inorganic Chemistry, 2016, 55(4): 1674.
[4] Algar W R，Kim H，Medintz I L，et al. Coordination Chemistry Reviews，2014，263-264(1): 65.
[5] Chaudhry C，Davis J，Yong Z，et al. Analytical Biochemistry，2016，497: 8.
[6] Lin G F，Liu T C，Hou J Y，et al. Journal of Fluorescence，2015，25(2): 361.
[7] Rossant C J，Matthews C，Neal F，et al. Journal of Biomolecular Screening，2015，20(4): 508.
[8] Nrskovlauritsen L，Thomsen A R，Brunerosborne H. International Journal of Molecular Sciences，2014，15(2): 2554.
[9] Wang J，Liu H，Huang X，et al. Microchimica Acta，2016，183(2): 749.
[10] Farino Z J, Morgenstern T J, Vallaghe J, et al. Plos One, 2016, 11(2): e0148684.
[11] Einhorn L, Krapfenbauer K. EPMA Journal, 2015, 6(1): 23.
[12] Cohen N, Mechaly A, Mazor O, et al. Journal of Fluorescence, 2014, 24(3): 795.
[13] Lehmusvuori A, Tapio A H, Mki-Teeri P, et al. Analytical Biochemistry, 2013, 436(1): 16.
[14] Xu H，Cao C S，Kang X M，et al. Dalton Transactions，2016，45(45): 18003.
[15] Zwier J M, Bazin H, Lamarque L, et al. Inorganic Chemistry, 2014, 53(4): 1854.
[16] Secundo F，Bacigalupo M A，Scalera C，et al. Journal of Food Composition and Analysis, 2012, 25(2): 221.
[17] Santos J G, Dutra J D L, Alves Junior S, et al. Journal of the Brazilian Chemical Society, 2013, 24(2): 236.
[18] Reddy D R, Pedró Rosa L E, et al. Bioconjugate Chemistry, 2011, 22(7): 1402.
