Discrimination of Common Wild Mushrooms by FTIR and Two-Dimensional Correlation Infrared Spectroscopy
MA Dian-xu1, LIU Gang1*, OU Quan-hong1, YU Hai-chao1, LI Hui-mei1, SHI You-ming2
1. School of Physics and Electronic Information, Yunnan Normal University, Yunnan Key Lab of Optoelectronic Information, Kunming 650500, China
2. School of Physics and Electronic Engineering, Qujing Normal University, Qujing 655011, China
Abstract:Fourier Transform Infrared Spectroscopy combined with correlation analysis, second derivative infrared spectroscopy and two-dimensional correlation infrared spectroscopy was used to discriminate 13 species of wild mushrooms. The results showed that the general characteristics of absorption bands in the original spectra were similar, which were mainly composed of protein and polysaccharides. Correlation analysis was applied for the spectra of samples, and the correlation coefficient between Termitomyces eurrhizus and Tylopilus plumbeoviolaceoides was 0.779, which was the minimum, while the correlation coefficient between Pleurotus lignatilis Gill and Hygrophorus lucorum Kalchbr was 0.960, which was the maximum. Coprinus comatus had little correlation with other samples. The differences of intensity, position and shape were observed in second derivative spectra in the range of 1 700~1 400 and 1 400~800 cm-1. In two-dimensional correlation spectra, the differences of the position or form of auto-peaks and cross peaks have been found in the range of 1 380~1 680 cm-1. The number, position and intensity of auto-peaks and cross peaks were obviously different in the range of 920~1 230 cm-1. The study demonstrates that Fourier Transform Infrared spectroscopy combined with correlation analysis, second derivative infrared spectroscopy and two-dimensional correlation infrared spectroscopy might be developed as a rapid method to discriminate different kinds of mushrooms.
马殿旭,刘 刚,欧全宏,于海超,李会梅,时有明. 常见野生蘑菇的红外光谱及其二维相关红外光谱的鉴别[J]. 光谱学与光谱分析, 2018, 38(07): 2113-2122.
MA Dian-xu, LIU Gang, OU Quan-hong, YU Hai-chao, LI Hui-mei, SHI You-ming. Discrimination of Common Wild Mushrooms by FTIR and Two-Dimensional Correlation Infrared Spectroscopy. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(07): 2113-2122.
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