Feasibility of 2DCOS Based on ATR-MIR in Surimi Quality Inspection
YOU Zhao-hong1，2, HONG Han-mei2, CHENG Fang2*, YANG Xiao-ling3
1. College of Life Sciences, Zaozhuang University, Zaozhuang 277000, China
2. College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China
3. College of Engineering, Jiangxi Agricultural University, Nanchang 330045, China
Abstract：The feasibility of 2DCOS (two-dimensional correlation spectroscopy) in the quality inspection of surimi was tested in this study.The changes of silver carp surimi protein secondary structure induced by freeze-thaw treatment were studied in suit using 2DCOS combined with ATR-MIR (attenuated total reflectance mid-infrared). The results showed that freeze-thaw cycles accelerated the oxidation process of surimi protein. The content of α-helix and intramolecular aggregated β-sheet all decreased, however the content of the anti-parallel β-sheet increased. 2DCOS analysis under the external perturbation of freeze-thaw cycles showed the change order was: α-helix, intramolecular aggregated β-sheet→antiparallel β-sheet→carbonyl. 2DCOS analysis under the perturbation of temperature showed that the protein secondary structure has been seriously damaged after three freeze-thaw cycles. And the effect of temperature on the carbonyl group of proteins was much smaller than that of the freeze-thaw cycles. These results indicated that the 2DCOS could explore and reflect the change degree of surimi protein. If these changes can be quantified, the 2DCOS could be used to detect the freshness of surimi.
基金资助: the National Natural Science Foundation of China (61573309)，the doctoral research start-up foundation of Zaozhuang University (2016BS05)
通讯作者:
成 芳
E-mail: fcheng@zju.edu.cn
作者简介: YOU Zhao-hong, (1979—)，female, lecturer, College of Life Sciences, Zaozhuang University e-mail: zijing1979@163.com
引用本文:
由昭红，洪寒梅，成 芳，杨小玲. 基于二维相关红外光谱的鱼糜品质检测可行性分析[J]. 光谱学与光谱分析, 2018, 38(05): 1626-1632.
YOU Zhao-hong, HONG Han-mei, CHENG Fang, YANG Xiao-ling. Feasibility of 2DCOS Based on ATR-MIR in Surimi Quality Inspection. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(05): 1626-1632.
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