Effects of Cuprum Stress on Position of Red Edge of Maize Leaf Reflection Hyperspectra and Relations to Chlorophyll Content
LI Yuan-xi1, 3, 5, CHEN Xi-yun1, 2* ，LUO Da1, 4, 5, LI Bo-ying1, WANG Shu-ren1, ZHANG Li-wei1
1. State Key Laboratory of Earth Surface Processes and Resource Ecology，Faculty of Geography, Beijing Normal University, Beijing 100875, China
2. Beijing Key Laboratory of Environmental Remote Sensing and Digital Cities, Beijing 100875, China
3. Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing 100101, China
4. Institute of Earth Environment, Chinese Academy of Sciences, Xi’an 710061, China
5. University of Chinese Academy of Sciences, Beijing 100049, China
Abstract：The effects of Cuprum stress on reflectance spectra and chlorophyll content of maize leaves were studied by corn planting under 5 different Cu2+ concentration treatment. During the indoor experiment, hyperspectral reflectance and the corresponding contents of chlorophyll of leaves of maize seedling were measured and their changing trends and relationships between Cu2+ concentration, chlorophyll content and red edge position (wavelength of reflection spectra) were analyzed. Results showed that the corn leaf reflection spectra had the obvious “blue shift of red edge” phenomenon, namely the red edge position of leaf spectra moved to short wave. The red edge position significantly correlated to Cuprum concentration (R=0.76), i. e. the blue shift of red edge increased with Cuprum concentration. In the meanwhile red edge blue-shifts increased with the extension of stress time. The chlorophyll a, chlorophyll b content and their ratio (Chla/Chlb) were significantly different between the five stress treatments (p=0.002, 0.007, 0.001). The content of chlorophyll covaried with Chla/Chlb. At the same time, Chla/Chlb and the concentration of Cuprum in the culture solution showed negative and significant correlation (r=-0.898), whilst, Chla/Chlb was significant and positively correlated with the mean red edge position (r=0.814). These results indicated that the blue shift of red edge of the reflectance spectra in the leaves of maize caused by Cuprum stress should attributed to the increase of chlorophyll b relative to chlorophyll a, which changed the leaf absorption spectrum and altered the red edge position of leaf reflectance.
Key words：Cuprum concentration; Maize; Reflection hyperspectra; Blue shift of red edge; Chlorophyll
李苑溪，陈锡云，罗 达，李波莹，王姝人，张力玮. 铜胁迫下玉米叶片反射光谱的红边位置变化及其与叶绿素的关系[J]. 光谱学与光谱分析, 2018, 38(02): 546-551.
LI Yuan-xi, CHEN Xi-yun ，LUO Da, LI Bo-ying, WANG Shu-ren, ZHANG Li-wei. Effects of Cuprum Stress on Position of Red Edge of Maize Leaf Reflection Hyperspectra and Relations to Chlorophyll Content. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(02): 546-551.
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