Abstract：In the present research we used gas chromatography-mass spectrometry (GC-MS)to determine metabolites of toluene diisocyanate (TDI) in mice and deduce the pathway for toluene diisocyanate metabolism in the organism. Conditions for TDI chromatography: Supelco PTETM-5 chromatographic column (30 mm×0.25 mm×0.25 μm); initial column temperature: 90℃, which was maintained for 30 min, then the temperature was increased at a rate of 40℃·min-1 to 280℃, and maintained for 5.25 min; temperature for the vaporizing chamber: 250℃; carrier gas: helium flowing at 1.0 μL·min-1. Conditions for chromatography of TDI metabolites in the organism: 94% methyl, 4% ethenyl-bonded-phase fused-silica capillary column (30+2 mX0.25 + 0.02 mm); initial column temperature: 30℃, which was maintained for 5 min, after and then was increased at a rate of 80℃·min-1 to 280℃, and maintained for 5 min; temperature for the vaporizing chamber: 250℃; carrier gas: helium flowing at 1.0 μL·min-1. Conditions for mass spectrometry: EI for ionization; 70 eV for ionization energy; 280℃ for connecting tube temperature; 35-350 μ for range of scanning; and 1.0 μL for sample size. The results showed that 2,4-toluene diisocyanate was metabolized into 2,4-diaminotoluene. Under the conditions selected for GC-MS, TDI metabolites in the organism can be isolated and identified.
季宇彬,汲晨锋*,邹翔,刘慧鑫. 气相质谱法对小鼠体内甲苯二异氰酸酯代谢产物的鉴定分析[J]. 光谱学与光谱分析, 2007, 27(09): 1886-1889.
JI Yu-bin, JI Chen-feng*, ZOU Xiang,LIU Hui-xin. Determination and Analysis of Toluene Diisocyanate Metabolites in Mice Using Gas Chromatography-Mass Spectrometry. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2007, 27(09): 1886-1889.
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