Essential Oils of Zingiberaceae Alpinia Kwangsiensis and Achasma Yunnanensis in Situ Research with Raman
SI Min-zhen1, 2, ZHANG De-qing1, 2, LI Lun1, 2, ZHANG Chuan-yun1, 2
1. Key Laboratory of Molecular Spectroscopy, Colleges and Universities in Yunnan Province, Chuxiong Normal University, Chuxiong 675000, China
2. Application Institute of Spectroscopy Technology, Chuxiong Normal University, Chuxiong 675000, China
Abstract：Non-destructive analysis of the essential oils ingredient in oil cells at room temperature was presented. This work showed the possibility to detect in situ the main components of the essential oils in Alpinia kwangsiensis and Achasma yunnanensis oil cells by means of Raman spectroscopy. Water slice of fresh samples were prepared. The oil cells in water slice can be seen using 20 objective lens of DXR Laser confocal micro Raman spectrometer. At the Alpinia kwangsiensis oil cell, high intensity bands were present in 1 638，1 600，1 555，1 203，1 001 cm-1，and low intensity bands were present in 1 716，1 577，1 496，1 407，1 346，1 307，1 273，1 181，1 156，1 029，958，618，218 cm-1. Together 26 spectroscopic bands were obtained. The Raman spectrum of the Methyl cinnamate, together 29 spectroscopic bands, were obtained. It was found that the 22 presented spectroscopic bands of Alpinia kwangsiensis oil cell correlated very well with those obtained by the Methyl cinnamate；At the Achasma yunnanensis oil cell, high intensity bands were present in 1 648，1 639，1 607，1 174，842，836 cm-1，and low intensity bands were present in 1 292，1 244，1 235，1 204，631 cm-1. Together 24 spectroscopic bands were obtained. The Raman spectrum of the 4-Allyl anisole, together with 29 spectroscopic bands(between 300～1 700 cm-1) was obtained. It was found that the 23 presented spectroscopic bands of Achasma yunnanensis oil cell correlated very well with those obtained by the 4-Allyl anisole. The experimental work was supported by quantum chemical calculations at the B3LYP/6-311G level of theory. The essential oils ingredient in oil cells of Zingiberaceae samples can be detected directly and quickly without extraction. This method can be use to quality control and development research for zingiberaceae plant essential oil extraction.
Key words：Essential oils of Alpinia kwangsiensis；Essential oils of Achasma yunnanensis；Raman spectrum；Methyl cinnamate；4-Allyl anisole；Density Functional Theory
司民真，张德清，李 伦，张川云. 姜科植物长柄山姜及茴香砂仁精油原位拉曼光谱研究[J]. 光谱学与光谱分析, 2018, 38(02): 448-453.
SI Min-zhen, ZHANG De-qing, LI Lun, ZHANG Chuan-yun. Essential Oils of Zingiberaceae Alpinia Kwangsiensis and Achasma Yunnanensis in Situ Research with Raman. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(02): 448-453.
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