Abstract:A new method using Surface enhanced Raman spectroscopy (SERS) was established for the rapid detection of Nafcillin residues in duck meat, and Au nanoparticles were used as enhancement substrate while NaCl solutions were used as active agent. In this research, the SERS characteristic peaks of Nafcillin solutions as well as their vibrational assignment were analyzed. Then, the SERS characteristic peaks of Nafcillin in duck extract were analyzed and determined for the identification of Nafcillin residues in duck meat,and the Raman intensities of nafcillin residues in duck meat extracted at 521 and 1 449 cm-1 were applied to optimize the detection conditions. Lastly, the internal standard method was used to quantitatively analyze the nafcillin residues in duck meat. It was revealed that 4 calibration curves built by Raman peaks intensities ratio had a good linearity when the residues concentrations of nafcillin in duck were 0.2~10 mg·L-1, and the determination coefficients were larger than 0.95. Three of the calibration curves using Raman peaks intensities ratio had superior accuracy with the recoveries of 88%~144%. The results showed the detection of nafcillin residues in duck meat using SERS was feasible, and the method was simple, rapid and accurate providing the technical support for detect the nafcillin residues in poultry meat food.
彭义杰,刘木华,赵进辉,袁海超,李 耀,陶进江,郭红青. 应用表面增强拉曼光谱法检测鸭肉中萘夫西林残留的研究[J]. 光谱学与光谱分析, 2017, 37(12): 3736-3742.
PENG Yi-jie, LIU Mu-hua, ZHAO Jin-hui, YUAN Hai-chao, LI Yao, TAO Jin-jiang, GUO Hong-qing. Study on Detection of Nafcillin Residues in Duck Meat Using Surface Enhanced Raman Spectroscopy. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2017, 37(12): 3736-3742.
[1] ZHU Zi-ying, GU Ren-ao, LU Tian-hong(朱自莹, 顾仁敖, 陆天虹). Application of Raman Spectroscopy in Chemistry(拉曼光谱在化学中的应用). Shengyang:Northeastern University Press(沈阳:东北大学出版社), 1998. 71.
[2] JI Wei(吉 薇). Studies on the Detection of Chloramphenicol and Its Sodium Succinate by Surface-Enhanced Raman Scattering(表面增强拉曼光谱测定氯霉素及其琥珀酸钠的研究). Jiangsu: Jiangnan University(江苏:江南大学), 2013. 1.
[3] SUN Mei-juan, TAO Zhan-hua(孙美娟, 陶站华). Journal of Guangxi Teachers Education University·Natural Science Edition(广西师范学院学报·自然科学版), 2014, 31(4): 27.
[4] WU Ya-dong, XU Wei-ping, JIN Qin-yu, et al(吴亚东, 徐维平, 金勤玉, 等). Guangzhou Chemical Industry(广州化工), 2015, 43(4): 17.
[5] LIU Yu-xing, JIAN Gui-qin, HE Xi-wen, et al(刘雨星, 菅桂芹, 何锡文, 等). Chinese Journal of Analytical Chemistry(分析化学), 2013, 41(2): 161.
[6] WU Qiang, HE Sheng-hu, CHEN Juan, et al(吴 强, 何生虎, 陈 娟, 等). Progress in Veterinary Medicine(动物医学进展), 2015, 36(6): 64.
[7] Bailón-Pérez M I, García-Campaa A M, del Olmo-Iruela M, et al. Journal of Chromatography, A, 2009, 1216(47): 8355.
[8] Ren Z Q, Zhang W D, Li J, et al. Journal of Chemical & Engineering Data, 2010, 55(8), 2687.
[9] TAO Xin-chen, HUANG He, LIAO Jian-meng, et al(陶昕晨, 黄 和, 廖建萌, 等). Journal of Chinese Institute of Food Science and Technology(中国食品学报), 2014, 14(1): 232.
[10] LIU Ping, HE Bao-xiang, HUANG Yun-fei(刘 平, 何宝祥, 黄云飞). The Rapid Diagnostic Stripe of the Candida Albicans in Cow Mastitis: (Chinese Patent)(奶牛乳房炎白色念珠菌快速诊断试纸条, 中国专利),201310674116.9. 2013-12-11.
[11] CHENG Jing-quan, YAO Su-wei(程敬泉, 姚素薇). Rare Metal Materials and Engineering(稀有金属材料与工程), 2012, 41(2): 348.
[12] WANG Lan, HUANG Shao-hua, HUANG Zu-fang et al(王 兰,黄少华,黄祖芳,等). The Journal of Light Scattering(光散射学报),2014, 26(1):13.
[13] WANG Shi, CHENG Jie, SU Xiao-ou(王 石,程 劼,苏晓鸥). Scientia Agricultura Sinica(中国农业科学),2014,47(11): 2224.
[14] ZHANG Quan, WENG Yu-xiang(张 权,翁羽翔). The Journal of Light Scattering(光散射学报),2015, 27(1):1.
[15] ZHAO Jin-hui, YUAN Hai-chao, HONG Qian, et al(赵进辉, 袁海超, 洪 茜, 等). Journal of Food Safety and Quality(食品安全质量检测学报), 2014, 5(3): 703.
[16] KANG Qian-qian, ZHOU Guang-ming(康倩倩, 周光明). The Journal of Light Scattering(光散射学报), 2011, 23(4): 368.
[17] Foteli W G, Lindsay F P, Bentley F F. Characteristic Raman Frequency of Organic Compounds(有机化合物的特征拉曼频率). Beijing: China Chemical Publishing House(北京:中国化学出版社), 1980. 93, 143.
[18] XU Yang, LUO Hai-xia, FAN Qiang, et al(徐 洋, 罗海霞, 范 强, 等). Contemporary Chemical Industry(当代化工), 2015, 44(4): 699.
[19] GAO Xiao-ling, Butler J S(高小玲, 巴特勒 J S). Chinese Journal of Analysis Laboratory(分析试验室), 1995, 14(4): 52.