The Common and Variation Peak Ratio Dual Index Sequence Analysis on UV Fingerprint Spectra of Paeonia Lactiflora Pall.
ZOU Hua-bin1, YUAN Hao2, WANG Ai-wu3, YUAN Jiu-rong2*, YUE Chun-hua2
1. School of Chemistry and Chemical Engineering of Shandong University (East Campus), Ji’nan 250100 China 2. College of Traditional Chinese Medicine, Shandong University of Traditional Chinese Medicine, Ji’nan 250014, China 3. The research center of natural pharmaceutical of Shandong Province Hospital, Ji’nan 250021, China
Abstract:Baishao and Chishao (Paeonia Lactiflora Pall.) and their close relative Danpi (Paeonia Suffruticosa Andr) samples were estimated quantitatively, based on their UV fingerprint spectra of the extracts obtained with chloroform, ethanol and water, by applying the common and variation peak ratio dual index sequence analysis method. The analytical results showed that the Baishao samples B2,B3 and B4 from the closest regions were the most similar samples. Their common peak ratios were larger than 70 percent and their variation peak ratios were less than 33.3 percent. However, there existed obvious differences among Baishao sample group 1(B1 and B5), group 2(B2,B3 and B4) and group 3(B6) from different regions. The common peak ratios among group 1(B1 and B5) and group 2(B2,B3 and B4) were lower than 60 percent, while those among group 1(B1 and B5) and group 3(B6) were less than 57 percent. The Baishao samples B1 and B5 from the same region collected in different years were of significant disparity, their common peak ratio was only 44.4 percent, but their variation peak ratios were larger than 100 percent. In fact, this method reaches the limitation of quantitative identification of herbs, and can distinguish at least two samples quantitatively.
Key words:UV fingerprint spectra;Dual index sequence analysis;Quantitative estimate;Paeonia Lactiflora Pall.
邹华彬1,袁浩2,王爱武3,袁久荣2*,岳春华2 . 白芍紫外指纹图谱共有峰率和变异峰率双指标序列分析[J]. 光谱学与光谱分析, 2007, 27(09): 1815-1819.
ZOU Hua-bin1, YUAN Hao2, WANG Ai-wu3, YUAN Jiu-rong2*, YUE Chun-hua2. The Common and Variation Peak Ratio Dual Index Sequence Analysis on UV Fingerprint Spectra of Paeonia Lactiflora Pall. . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2007, 27(09): 1815-1819.
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