Fluorescence Microscopic Study of Environmental Water Sample by a Self-Ordered Ring Technique with Microwave Heating
LIU Ying1,2,HUANG Cheng-zhi1*
1. College of Pharmacy, Southwest University, Chongqing 400715, China 2. College of Chemistry and Environmental Science, Inner Mongolia Normal University, Huhhot 010022, China
Abstract：A self-ordered ring (SOR) technique with microwave heating is proposed for the environmental water samples based on the capillary flow of solvent on the solid surface of glass slide support. In the reaction medium of pH 4.58 HAc-NaAc containing poly (vinyl alcohol)-124, a SOR of berberine with the outer diameter of 1.1 mm and the ring belt width of 19.2 μm can be formed using microwave heating and its fluorescence was quenched by picric acid. The maximum fluorescence intensity (Imax) was found to be proportional to picric acid content. When a 0.1 μL droplet of berberine-picric acid mixture was spotted on the solid surface, picric acid in the range of 1.3-30.0×10-7 mol·L-1 can be detected, and the limit of detection can reach 1.3×10-8 mol·L-1. With the present method, the contents of picric acid in natural water samples and synthetic samples were satisfactorily determined with its recoveries of 96.3％-108.0％ and RSD lower than 3.3%. The sensitivity enhanced 50 times than that by spot analysis, and 60 times than that in solution. Moreover, the interference from background was reduced greatly. The authors believe that the SOR technique will find wide practicability and superiority in both environment and biochemistry.
Key words：Picric acid;Berberine;Self-ordered ring (SOR) technique;Fluorescence microscopic analysis
刘颖1,2,黄承志1*. 微波加热自组装成环荧光显微成像技术在环境水样中的应用研究[J]. 光谱学与光谱分析, 2007, 27(09): 1802-1806.
LIU Ying1,2,HUANG Cheng-zhi1*. Fluorescence Microscopic Study of Environmental Water Sample by a Self-Ordered Ring Technique with Microwave Heating. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2007, 27(09): 1802-1806.
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