Discrimination of Fritillariae thunbergii Bulbus Treated by Sulfur Fumigation Based on NIR Coupled with PLS-DA
HE Juan1, ZHAO Yan-ru2, HE Yong2*
1. Zhejiang Academy of Traditional Chinese Medicine, Key Laboratory of Research and Development of Chinese Medicine of Zhejiang Province, Hangzhou 310007, China
2. College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China
Abstract：Fritillariae thunbergii Bulbus，as one of the famous “Zhe Ba Wei”，is often used as expectorant and antitussive medicine. Sulfur fumigation is commonly good for whitening, mothproofing and extending the shelf-life of medicines. However, excessive sulfur fumigation will affect the quality of traditional Chinese medicines, even damage human health. Therefore, using non-destructive detection method of Fritillariae thunbergii Bulbus with sulfur fumigation is beneficial to monitor the quality of traditional Chinese medicines. This paper used near infrared spectroscopy (NIR) (900～1 700 nm) combined with chemometrics to detect the Fritillariae thunbergii Bulbus fumigated with different concentrations of sulphur. “Boxplot” was adopted to analyze the difference among the samples in the range of 1 000～1 100 nm. Principal component analysis (PCA) was also used to cluster analysis of six different samples. Then successive projection algorithm (SPA) was applied to extract ten different characteristic wavelengths and establish the partial least squares-discriminant analysis (PLS-DA) models. Results showed that PLS-DA model had the similar results with full spectra analysis. Furthermore, NIR combined with chemometrics can be used to analyze the Fritillariae thunbergii Bulbus fumigated with different concentrations of sulphur. It provided a theoretical reference for the analysis of the quality of Fritillariae thunbergii Bulbus and the design of the portable instruments.
何 娟，赵艳茹，何 勇. 近红外光谱结合偏最小二乘判别对硫熏浙贝母的无损鉴别[J]. 光谱学与光谱分析, 2017, 37(10): 3070-3073.
HE Juan, ZHAO Yan-ru, HE Yong. Discrimination of Fritillariae thunbergii Bulbus Treated by Sulfur Fumigation Based on NIR Coupled with PLS-DA. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2017, 37(10): 3070-3073.
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