Monitoring of Photodynamic Therapy of Port Wine Stain by Fluorescence Spectroscopy
WANG Lei1, GU Ying1*, LI Xiao-song1, LIU Fan-guang1, YU Chang-qing2
1. Department of Laser Medicine, Chinese PLA General Hospital, Beijing 100853, China 2. Department of Optical Engineering, Beijing Institute of Technology, Beijing 100081, China
Abstract：The blood drug level and the formation of photoproduct were monitored during photodynamic therapy (PDT) of port wine stain (PWS) by fluorescence spectroscopy. The irradiation was implemented by a 532nm double-frequency Nd∶YAG laser, and the collection of fluorescence spectra was completed with the use of spectrograph and ICCD. In the experiment for validation of the system, the fluorescence basis spectra of hematoporphyrin monomethyl ether (HMME)-sensitized mouse normal skin were constructed, and, by least-square fitting, HMME fluorescence (624nm) could be discriminated from that of photoproduct (652nm). The fitting of fluorescence spectra measured from PWS patients skin containing PSD-007 was performed with the same basis spectra as those from the mouse skin. The curves of blood drug level of different patients with significant variance, as well as those of formation and bleaching of photoproduct were obtained. Fluorescence spectroscopy monitoring system and fitting method presented here can provide technical means for rigorous quantitative PDT dosimetry method, and the results obtained here will make for the individual scheme of PDT.
Key words：Photodynamic therapy;Dosimetry;Fluorescence specrum;Port wine stain;Blood drug level;Photoproduct
王 雷1,顾瑛1*,李晓松1,刘凡光1,于常青2. 鲜红斑痣光动力治疗的荧光光谱监测[J]. 光谱学与光谱分析, 2007, 27(09): 1788-1792.
WANG Lei1, GU Ying1*, LI Xiao-song1, LIU Fan-guang1, YU Chang-qing2 . Monitoring of Photodynamic Therapy of Port Wine Stain by Fluorescence Spectroscopy. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2007, 27(09): 1788-1792.
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