Abstract:In the clinic, for the diagnosis of a variety of major diseases, it has great significance in terms of quick detection of concentration of human serum albumin (HSA). However, there are bulky, long test cycle, complex operations and other shortcomings for common medical detection equipment. It will have a very broad application prospects as for the design of a fast, high-precision portable detection system for HSA concentration. HSA concentration detection system was designed based on Resonance Rayleigh Scattering(RRS) spectroscopy. The mixed solution was generated with HSA and Tetraamino-phthalocyanine copper (CuTAPc) reaction, and it had a RRS enhancement effect at 475.0 nm, whereby the function of RRS intensity and HSA concentration was constructed. In the experiment, ultraviolet semiconductor lasers and 475.0 nm narrowband filter was used, the scattered light signal was collected with high gain photo-detector. Different concentrations of HSA were respectively detected with different concentrations of CuTAPc. Experimental results show that response voltage will increase significantly with the increasing of concentrations of HSA,but it is not sensitive to concentration changes for CuTAPc; It basically meet the linear change by the response voltage from scattered light and the concentration of HSA in the range from 0.1 to 1.0 μg·mL-1, and it meets the design requirements of rapid detection.
Key words:Resonance Rayleigh scattering (RRS); Human serum albumin (HSA); Tetraaminophthalocyanine copper (CuTAPc); Linear fitting
李 沅,李 凯,赵冬娥. 基于RRS的人血清白蛋白快速检测系统设计[J]. 光谱学与光谱分析, 2017, 37(06): 1925-1929.
LI Yuan, LI Kai, ZHAO Dong-e. Rapid Detection System for HSA Based on RRS. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2017, 37(06): 1925-1929.
[1] Liu Y W, Liu C W, Chang H T. Talanta, 2011, 84(2): 324.
[2] Mohd, Ayaz. Journal of Fluorescence, 2011, 4(21): 1357.
[3] MA Lu, WEN Gui-qing, LIU Qing-ye(马 璐,温桂清,刘庆业). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2014, 34(9): 2481.
[4] David J Perry, David A Fitzmaurice, Steve Kitchenl. British Journal of Haematology, 2010,(150): 501.
[5] ZHANG Min, WU Li-min, ZHOU Shang(张 敏, 吴利敏, 周 尚). Journal of Instrumental Analysis(分析测试学报),2012, 6: 8.
[6] Axel Warsinke. Anal. Bioanal. Chem., 2009, 393: 1393.
[7] Ge S, Tang W, Han H, et al. J. Chromatogr. A, 2013, 1295(6): 128.
[8] Liu Jinfang, Li Nianbing, Luo Hongqun. Spectrochimica Acta, 2011, (79): 631.
[9] Ali Mohd Sajid. Journal of Colloid and Interface Science, 2010, 2(352): 436.
[10] PANG Xiang-dong, ZHENG Xiao-feng, DENG Qin-xin(庞向东, 郑晓锋, 邓钦心). Journal of Analytical Science(分析科学学报). 2011, 2: 84.
[11] Jochen S, Andra E T. Anatom. Record, 2012, 295(71): 1837.
[12] Anglstier J, Stenibegr I Z. J. Chem. Phys., 1981, 74(2): 786.