Discriminate the Rape Sclerotinia at Early Stage Based on Confocal Raman Spectroscopy
ZHAO Yan-ru1, LI Xiao-li1, YU Ke-qiang2, CHENG Fan3, LIU Ji-qiang4, HE Yong1*
1. College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China 2. College of Mechanical and Electronic Engineering, Northwest A&F University, Yangling 712100, China 3. Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China 4. Second Institute of Oceanography of the State Oceanic Administration, Hangzhou 310012, China
Abstract：Oilseed rape(Brassica napus L. ) is a principal source of edible oil for human consumption and it feeds livestock as a by product with high energy and protein. However, oilseed plants often suffer from the invasion of various diseases, which could affect the yield and quality of the rapeseeds. Rape sclerotinia rot caused by the fungus sclerotinia sclerotiorum (Lib. ) de Bary may severely affect the growth of oilseed rape. Therefore, searching non-invasive detection methods of detection plant disease at early stage is crucial for monitoring growing conditions of crops. Confocal Raman spectroscopy in the region of 500～2 000 cm-1 coupled with chemometrics methods were employed to discriminate the rape sclerotinia disease at early stage on the oilseed rape leaves. A total of 60 samples(30 healthy plant leaves and 30 infected leaves) were used to acquire the Raman spectra and wavelet transform was applied to remove the fluorescence background. Regression coefficients of the partial least squares-discriminant analysis(PLS-DA) were used to select the 8 characteristic peaks based on the whole Raman spectra. 983，1 001，1 205，1 521，1 527，1 658，1 670 and 1 758 cm-1 were employed to establish PLS-DA discriminate models and recognition accuracy was 100%. The results showed Raman spectra combined with chemometrics method is promising for detecting rape sclerotinia infection in the oilseed rape leaves at early stage. This study provided a theoretical reference for researching the interaction between the fungus and plants and early detecting of disease infection.
赵艳茹1，李晓丽1，余克强2，程 帆3，刘吉强4，何 勇1* . 基于共聚焦拉曼光谱技术的油菜菌核病早期判别分析 [J]. 光谱学与光谱分析, 2017, 37(02): 467-471.
ZHAO Yan-ru1, LI Xiao-li1, YU Ke-qiang2, CHENG Fan3, LIU Ji-qiang4, HE Yong1* . Discriminate the Rape Sclerotinia at Early Stage Based on Confocal Raman Spectroscopy. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2017, 37(02): 467-471.
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