The Antioxidant Inhibition of Clove Effective Fraction on Lipid, Protein and Spectra Variation of LDL
YANG Qiong-yu1, WAN Yan1,4, JIANG Shen-hua1,2,3,4*, HAO Shu1,4, ZENG Jun-jie1, ZHANG Hua-hao1,4, MA Hai-le1,2, QU Wen-juan1,2, SHEN Yong-gen1,3
1. School of Pharmacy and Life Science, Jiujiang University, Jiujiang 332000, China 2. School of Food and Biological Engineering, Jiangsu University, Jiangsu Provincal Key Lab of Physical Processing of Agricultural Products, Zhenjiang 212013, China 3. College of Food Science and Engineering, Jiangxi Agricultural University, Jiangxi Key Laboratory of Natural Products and Functional Foods, Nanchang 330045, China 4. Jiujiang Andehe Biotechnology Co., Ltd., Jiujiang 332000, China
摘要： LDL氧化修饰是致动脉粥样硬化的关键性因素。其中，在氧化修饰过程中LDL所含脂类、蛋白及其光谱学性质均会受到影响。前期研究结果发现丁香对LDL氧化修饰具有很强的抑制作用。但是，丁香是否是通过抑制脂类及蛋白氧化来实现，以及丁香是否能抑制LDL氧化修饰后光谱学性质的改变，这些问题目前均不清楚。据此，运用紫外吸收光谱、荧光光谱等方法研究了丁香有效部位(effective fraction from clove，EFC)对LDL氧化修饰的抑制效果。研究结果表明，EFC能有效延缓脂质氧化过程中共轭二烯(CD)增殖及推后其达到最大值，并抑制胆固醇降解;也可抑制蛋白组分apoB100中Trp荧光猝灭及Lys氧化修饰和荧光光谱变化;同时也能抑制LDL中脂质与蛋白过氧化产物-脂褐素的生成;此外，该部位对LDL 氧化修饰过程中紫外-可见光谱学特征的改变也有影响。论文将为丁香抗AS功能食品研发提供参考。
关键词：丁香;有效部位;低密度脂蛋白;荧光光谱;紫外-可见光谱
Abstract：Oxidation of low density lipoprotein (LDL) has been considered as the critical factor which led to atherosclerosis (AS). Lipid and protein in LDL were oxidized to cause change of spectra during oxidation. Clove has been demonstrated to possess the strongest antioxidant capacity among 87 both medical and edible plants proclaimed by China. However, whether LDL oxidation is inhibited by clove? If so, whether it inhibits the oxidation of lipid and protein in LDL, and how does spectral characteristic of LDL change during oxidation when clove was added into LDL. Currently, these questions are still unclear. Therefore, the inhibition efficiency of the effective fraction from clove (EFC) on oxidation of LDL was studied by UV-Visible spectra and fluorescence spectra. The results indicated that EFC might effectively delay propagation of conjugated diene (CD) during LDL oxidation and postpone reaching maximum of its content. Likewise, it might inhibit cholesterol degradation, tryptophan (Trp) fluorescence quenching, lysine (Lys) residues from being modified, and lipofuscins from being generated in peroxidatic reaction among lipid and protein.Besides, EFC also might affect variation of UV-Visible spectra of LDL during oxidation. This study provides reference for future research and development of clove functional food inhibiting AS.
Key words：Clove;The effective fraction;Low density lipoprotein (LDL);Fluorescence spectra;UV-Visible spectra
杨琼玉1，万 严1,4，江慎华1,2,3,4*，郝 澍1,4，曾钧杰1，张化浩1,4，马海乐1,2，曲文娟1,2，沈勇根1,3 . 丁香有效部位对LDL中脂类、蛋白氧化修饰及光谱学变化的抑制 [J]. 光谱学与光谱分析, 2017, 37(01): 312-320.
YANG Qiong-yu1, WAN Yan1,4, JIANG Shen-hua1,2,3,4*, HAO Shu1,4, ZENG Jun-jie1, ZHANG Hua-hao1,4, MA Hai-le1,2, QU Wen-juan1,2, SHEN Yong-gen1,3. The Antioxidant Inhibition of Clove Effective Fraction on Lipid, Protein and Spectra Variation of LDL. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2017, 37(01): 312-320.
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