Abstract：The soluble lignin and furan compounds in hydrolysate of lignocellulose biomass have absorbed peaks and inter-superimpose in UV spectroscopy. Therefore, this paper developed a method for the determination of furfural and 5-hydroxymethyl furfural in ethanol-water refining liquors of lignocellulose biomass based on UV spectroscopy. The study showed that there is a good and linear relationship between maximum absorption (λmax) and the concentration percent of 5-HMF (W5-HMF) of M, the regression coefficient up to 0.998 4 (R2=0.998 4). Furthermore, a better linear relationship was also observed between the concentration of mixture (cM) and absorptivity (A) when the W5-HMF was constant, the R2 of 0.998 6～1.000 0 and reproducibility are desirable. The contents of F and 5-HMF could be calculated accurately according to the perfect liner relationship of A and cM, λmax and W5-HMF, and the relative deviation of F and 5-HMF of 5.6% and 3.7%, recovery of 89%～96%, RSD of 0.21%～0.85%, respectively. Overall, The simple rapid and precise method in this paper can be applied to the determination of the F and 5-HMF in the hydrolysate of ethanol-water refining lignocellulose biomass.
潘梦丽，平清伟*，张 健，李 娜 . 紫外光谱法快速测定生物质乙醇/水水解液中糠醛和5-羟甲基糠醛含量 [J]. 光谱学与光谱分析, 2017, 37(01): 146-149.
PAN Meng-li, PING Qing-wei*, ZHANG Jian, LI Na . Rapid Method for Determination of Furfural and 5-Hydroxymethyl Furfural of Ethanol-Water Hydrolysate of Biomass with UV Spectroscopy. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2017, 37(01): 146-149.
[1] Vida P, Manjusri M, Amar K M. BioResources, 2013, 8(4): 5083. [2] Aracri E, Díaz Blanco C, Tzanov T. Green Chem., 2014, 16: 2597. [3] Robert-Jan V P, Jong E D, Jan C W, et al. Chem. Rev., 2013, 113: 1499. [4] De S, Saha B, Luque R. Bioresource Technology, 2015, 178: 108. [5] Paraskevi Panagiotopoulou, Dionisios G. Vlachos，2014，480: 17. [6] Su Yu, Heather M Brown, Huang Xiwen, et al. Applied Catalysis A: General, 2009, 361: 117. [7] Jin Hua, Li Yanshuo, Liu Xinlei. Chemical Engineering Science，2015，124: 170. [8] Cai C M, Zhang Taiying, Kumar R, et al. Journal of Chemical Technology and Biotechnology, 2014, 89: 2. [9] Petisca C, Henriques A R, Pérez-Palacios T. Journal of Food Composition and Analysis，2014，33: 20. [10] Sefik Tunc M, Juben Chheda, Evert van der Heide. Industrial Engineering Chemistry Research 2013，52: 13209. [11] Ariffin A A, Ghazali H M, Kavousi P. Food Chemistry, 2014, 154: 102. [12] Albalasmeh A A, Berhe A A, Ghezzehei T A. Carbohydrate Polymers, 2013, (97): 253. [13] Hu S Q, Zhang Z F, Zhou Y X, et al. Green Chemistry, 2008, 10(12): 1280. [14] Xun Hu, Richard Gunawan, Daniel Mourant. Fuel, 2012; 97: 512. [15] Xun H, Shuai W, Westerhof R J M, et al. Fuel, 2014, 141: 56. [16] Clarkson S M, H-Brehm S D, Giannone R J, et al. Biotechnology for Biofuels, 2014, (7): 165. [17] Gkmen V，瘙塁enyuva H Z. Journal of Agricultural and Food Chemistry, 2006, 54(8): 2845. [18] Hyvrinen S, Mikkola J P, Murzin D Y. Catalysis Today，2014，223: 18. [19] Merike Vaher, Kati Helmja, Andres Ksper. Catalysis Today，2012，196: 34. [20] Swiss Food Manual, Kapitel Honig, Eidg. Druck und Materialzentrable，1995.
