交替惩罚三线性分解算法结合三维荧光光谱法同时分辨和测定酪氨酸、苯丙氨酸和色氨酸

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摘要：用交替惩罚三线性分解算法(APTLD)结合三维荧光光谱法给出的二维数据对酪氨酸、苯丙氨酸和色氨酸进行了同时定性定量分析，为直接同时测定混合氨基酸中此三种物质提供了一种新的分析方法, 其测定相关系数分别为0.9987，0.9995和0.9993。采用超声波组织细胞破碎法对木槿叶中的氨基酸进行提取，利用APTLD法对提取液中氨基酸进行定量测定，测定相对标准偏差分别为0.84％，0.36％，1.59％, 回收率分别在101.0％～92.7％, 106.5％～93.0％, 103.0％～95.0％之间，方法简洁、快速、准确可靠，结果令人满意。

关键词：交替惩罚三线性分解算法;三维荧光光谱;酪氨酸;色氨酸;苯丙氨酸

Abstract：A new method using alternating penalty trilinear decomposition algorithm coupled with excitation-emission matrix fluorometry has been developed for simultaneous resolution and determination of tyrosine, phenylalanine and tryptophan. Their correlation coefficients were 0.9987, 0.9995and 0.9993 respectively. The contents of tyrosine, phenylalanine and tryptophan in Hibiscus syriacus L. leaves were also be determined by this method after being extracted by ultrasonic. The coefficients of variation and the recoveries of the three amino acids were 0.84%, 0.36%, 1.59% and 101.0%-92.7%, 106.5%-93.0%, 103.0%-95.0% respectively. All these show that this is a simple, fast and cridible method.

Key words：Alternating penalty trilinear decomposition algorithm(APTLD);3D emission-excitation matrix fluorometry;Tyrosine;Tryptophan;Phenylalanine

收稿日期: 2006-03-28 修订日期: 2006-06-28

中图分类号:

O657.3

通讯作者: 任凤莲 E-mail: xj410001@yahoo.com.cn

引用本文:

肖劲,任凤莲^*,宋鸽,廖律,禹文峰,曾涛. 交替惩罚三线性分解算法结合三维荧光光谱法同时分辨和测定酪氨酸、苯丙氨酸和色氨酸[J]. 光谱学与光谱分析, 2007, 27(10): 2088-2092.
XIAO Jin, REN Feng-lian^*, SONG Ge, LIAO Lü, YU Wen-feng, ZENG Tao. Simultaneous Resolution and Determination of Tyrosine, Tryptophan and Phenylalanine by Alternating Penalty Trilinear Decomposition Algorithm Coupled with 3D Emission-Excitation Matrix Fluorometry . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2007, 27(10): 2088-2092.

链接本文:

https://www.gpxygpfx.com/CN/Y2007/V27/I10/2088

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