Examining the Effects of Biochar Application on Soil Phosphorus Levels and Phosphatase Activities with Visible and Fluorescence Spectroscopy
ZHANG Yu-lan1, CHEN Li-jun1*, ZHANG Yu-ge2, WU Zhi-jie1, MA Xing-zhu3, YANG Xiao-zhu1
1. Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China 2. Key Laboratory of Regional Environment and Eco-remediation, College of Environment, Shenyang University, Shenyang 110044, China 3. Institute of Soil Fertilizer and Environment Resource, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China
Abstract：Biochar could be got from crop straw which contain rich carbon under oxygen free or oxygen limited conditions at low temperature. The application of biochar into soil is beneficial to ease the pressure of handling straw, reduce pollution, reduce greenhouse gas emissions and improve soil quality. This study was carried out in a cornfield containing meadow brown soil at the lower reaches of Liao River which was treated with different amounts of biochar (0, 360, 1 800, 3 600 kg·ha-1) and fertilizer. We investigated the contents of soil available phosphorus (AP), organic P (OP) and total P (TP). We also investigated the enzyme activities of soil acid phosphatase (AcP), alkaline phosphatase (AlP) and phosphodiesterase (PD) via a fluorescence spectroscopy method by using a fluorescent conjugated polymer as the substrate. Soil AP contents increased drastically with the increasing application of biochar, whereas the OP and TP contents exhibited little change. The increase in AP contents was ascribed to the introduction of P into the soil via biochar. Soil AlP and PD activities increased with increasing biochar application. Soil AcP activity increased significantly after the application of the appropriate amount of biochar (1 800 kg·ha-1), whereas it was inhibited by the application of high levels of biochar (3 600 kg·ha-1), perhaps due to the intrinsic alkalinity of biochar. The effect of Biochar inputs on soil phosphorus element and phosphatase activity is the comprehensive embodiment of the soil physical properties, chemical properties, and microbial community structure and metabolic capacity. We should further study such item. The fluorescent microplate method used in this study has many advantages, such as accuracy, rapidness and simple to perform.
张玉兰1，陈利军1*，张玉革2，武志杰1，马星竹3，杨晓竹1 . 生物炭施用对土壤磷及磷酸酶活性影响的可见和荧光光谱法研究 [J]. 光谱学与光谱分析, 2016, 36(07): 2325-2329.
ZHANG Yu-lan1, CHEN Li-jun1*, ZHANG Yu-ge2, WU Zhi-jie1, MA Xing-zhu3, YANG Xiao-zhu1. Examining the Effects of Biochar Application on Soil Phosphorus Levels and Phosphatase Activities with Visible and Fluorescence Spectroscopy. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2016, 36(07): 2325-2329.
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