Rapid Determination of Klason Lignin Content in Bamboo by NIR
LI Gai-yun1,HUANG An-min1,WANG Ge2,QIN Dao-chun2,JIANG Ze-hui1*
1. Research Institute of Wood Industry, Chinese Academy of Forestry, Beijing 100091, China 2. International Center for Bamboo and Rattan, Beijing 100102, China
Abstract:Lignin is one of the main components of lignocellulosic materials. The main purpose of wood cooking and bleaching is to remove lignin by chemical agent in paper industry. Whereas the lignin content shows wide variations depending on its tree specie, site condition, part and so on, it is essential to analyze the lignin content of different raw material. The aim of this paper is to develop a rapid near infrared (NIR) reflectance spectroscopic method to characterize the Klason lignin content of bamboo. Fifty four samples from three growth years, two positions along the longitudinal directions and three positions along the radial directions within a bamboo pole were prepared. The Klason lignin contents of 54 samples were analyzed according to traditional chemical method, the spectra of these samples were collected by NIR in the range of 350 to 2500 nm, and the relationship between the lignin content and the spectra of these samples was established by multivariate statistical technique. After second derivative pretreatment of raw spectra, the Klason lignin contents of the bamboo samples were quantified using partial least-squares statistical analysis (PLS1) and full cross validation in the range of 1011-1675 nm and1930-2488 nm. High coefficients of correlation (r) were obtained between the predicted NIR results and those obtained from traditional chemical method. The correlation coefficient of calibration model and prediction model was 0.99 and 0.97, respectively. The standard error of calibration (SEC) and standard error of prediction (SEP) was 0.36% and 0.59%, respectively. It was found that the lignin content in bamboo could be determined rapidly with reasonable accuracy by the NIR method.
[1] YANG Shu-hui(杨淑蕙). Lignocellulosic Fiber Chemistry(植物纤维化学). Beijing: China Light Industry Press(北京:中国轻工业出版社), 2001. 7. [2] ZHANG Qi-sheng, GUAN Ming-jie, JI Wen-lan(张齐生,关明杰,纪文兰). Journal of Nanjing Forestry University(Natural Sciences)(南京林业大学学报·自然科学版), 2002, 26(2): 7. [3] Kelley S S, Rials T G, Snell R, et al. Wood Science and Technology, 2004, 38(4): 257. [4] Hodge G R, Woodbridge W C. Journal of Near Infrared Spectroscopy, 2004, 12(6): 381. [5] Martin M E, Aber J D. Journal of Near Infrared Spectroscopy, 1994, 2(1): 25. [6] Yamada T, YEH Ting-feng, CHANG Hou-min, et al. Holzforschung, 2006, 60(1): 24. [7] Hoffmeyer P, Pedersen J G. Holzals Roh-und Werkstoff, 1995, 53: 165. [8] Poke F S, Raymond C A. Journal of Wood Chemistry and Technology, 2006, 26(2): 187. [9] Poke F S, Wright J K, Raymond C A. Journal of Wood Chemistry and Technology, 2004, 24(1): 55. [10] Taylor A, Lloyd J. Forest Products Journal, 2007, 57(1-2): 116. [11] Acuna M A, Murphy G E. Forest Products Journal, 2006,56(11-12):67. [12] Jones P D, Schimleck L R, Peter G F, et al. Canadian Journal of Forest Research, 2005, 35(1): 85. [13] Defo M, Taylor A M, Bond B. Forest Products Journal, 2007, 57(5): 68. [14] Fackler K, Schwanninger M, Gradinger C, et al. FEMS Microbiology Letters, 2007, 271(2): 162. [15] Jones P D, Schimleck L R, Peter G F, et al. Wood Science and Technology, 2006, 40(8): 709. [16] Sykes R, Li B, Hodge G, et al. Canadian Journal of Forest Research, 2005, 35(10): 2423. [17] JIANG Ze-hui, HUANG An-min, WAN Bin(江泽慧, 黄安民, 王 斌). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2006, 26(6): 1034. [18] JIANG Ze-hui, HUANG An-min(江泽慧, 黄安民). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2006, 26(8): 1464. [19] JIANG Ze-hui, FEI Ben-hua, YANG Zhong(江泽慧, 费本华, 杨 忠). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2007, 27(3): 435. [20] JIANG Ze-hui, LI Gai-yun, WANG Ge, et al(江泽慧, 李改云, 王 戈, 等). Chemistry & Industry of Forest Products(林产化学与工业), 2007, 27(1): 15. [21] YANG Zhong, JIANG Ze-hui, FEI Ben-hua, et al(杨 忠, 江泽慧, 费本华, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2007, 27(4): 686.