Discrimination of Panax Notoginseng from Different Regions by UV Spectra Characteristics Combined with Chemometric Method
WANG Yuan-zhong1*, ZHONG Gui1, 2, ZHANG Ji1, ZHAO Yan-li1, YANG Tian-mei1, ZHANG Jin-yu1*
1. Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, Kunming 650200, China 2. Institute of Plant Nutrition and Fertilizer, Yunnan University of Nationalities, Kunming 650500, China
摘要： 三七[Panax notoginseng(Burkill) F. H. Chen]为我国传统名贵中药，其质量受生长环境影响。本研究采用紫外指纹图谱技术建立快速准确的定性方法，研究不同产地三七之间的关系。采集云南10个不同地区共50个三七样品的紫外光谱，通过3组平均、4点平滑和1阶求导对原始图谱进行优化处理;考察样品超纯水、95%乙醇和三氯甲烷提取液紫外光谱的吸收峰数目，确定最佳提取溶剂;比较三七紫外光谱特征，探讨不同产地样品间的差异;利用偏最小二乘判别分析(partial least square discrimination analysis, PLS-DA)对光谱数据进行处理，研究不同产地三七样品之间的关系。结果显示，样品三氯甲烷提取液紫外光谱的吸收峰数目最多，该方法的精密度、重复性和在30 h内稳定性的变异系数RSD%分别在0.00～0.42，0.00～0.54，0.00～0.60之间。不同产地三七样品的紫外光谱峰形相似，吸光度值具有差异，呈现一定指纹特性;主要共有峰为194，200，204，210和218 nm，吸光度分布在0.00～4.00之间，表明三七的主要成分组成与产地相关性低，含量与产地存在相关性。PLS-DA得分图直观显示了不同产地样品的分类情况及三七样品之间的关系，图谱相似的样品聚在较近区域，图谱有差异的样品区分较为明显，10个产地样品被分为四类。该方法能快速准确对不同产地三七样品进行定性分析，阐明样品紫外指纹图谱与产地的关系，为中药的资源鉴别提供理论参考。
关键词：三七;紫外光谱;化学计量学;资源鉴别
Abstract：The Panax notoginseng is an important Chinese herbal medicine (CHM) and the quality is affected by growing environment. UV spectra approach was used to study the relationships between UV spectra of P. notoginseng and locations, rapidly. The UV spectra of fifty P. notoginseng samples which collected from ten regions were obtained and processed by using mean value, smoothing and derivative. The numbers of common peaks of chloroform, ethanol and water extractions were compared, respectively and the best extraction solvent was confirmed. The differences and relationships of P. notoginseng samples from different locations were investigated by comparing the characteristics of UV spectra combined with partial least square discriminant analysis (PLS-DA). The results showed that the extract ratio was the best in chloroform and the RSD% of precision, repeatability and stability in 30 h were 0.00～0.42, 0.00～0.54 and 0.00～0.60, respectively. The spectra of samples collected from different areas were similar except the absorbance. It could display the fingerprint characteristics. The common peaks of UV spectra were 194, 200, 204, 210 and 218 nm and the peaks absorption value ranged from 0.00 to 4.00. It showed that the main constituent had low correlation with collection regions while the content may be affected by the collection areas. The PLS-DA score plots could show the relationships among the P. notoginseng from different locations. In conclusion, this qualitative method could evaluate P. notoginseng samples from different locations rapidly and exactly. Moreover, it could provide the reference for source discrimination of CHM.
王元忠1*，钟 贵1, 2，张 霁1，赵艳丽1，杨天梅1，张金渝1*. 紫外指纹图谱结合化学计量学对不同产地中药三七的鉴别研究[J]. 光谱学与光谱分析, 2016, 36(06): 1789-1793.
WANG Yuan-zhong1*, ZHONG Gui1, 2, ZHANG Ji1, ZHAO Yan-li1, YANG Tian-mei1, ZHANG Jin-yu1*. Discrimination of Panax Notoginseng from Different Regions by UV Spectra Characteristics Combined with Chemometric Method. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2016, 36(06): 1789-1793.
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