Spectral Analysis of Interaction between Human Telomeric G-Quadruplex and Liliflorin A, the First Lignan Derivative Interacted with G-Quadruplex DNA
LIU Ting-ting1, ZHOU Shuang1, JIA Qian-lan1, WANG Wen-shu1,2*, YAN Xiao-qian1, ZHANG Wen-hao3, WANG Shuai-qi1, JIAO Yu-guo1
1. College of Life and Environmental Sciences, Minzu University of China, Beijing 100081, China 2. Beijing Engineering Research Center of Food Environment and Health, Minzu University of China, Beijing 100081, China 3. Center of Biomedical Analysis, Tsinghua University, Beijing 100084, China
Abstract：Human telomeric G-quadruplex is a four-stranded structure folded by guanines (G) via Hoogsteen hydrogen bonding. The ligands which stabilize the G-quadruplex are often telomerase inhibitors and may become antitumor agents. Here, the interaction between a lignan derivative liliflorin A and human telomeric sequence dGGG(TTAGGG)3G-quadruplex HTG21 were examined by CD, FRET, and NMR spectroscopic methods. In addition, Molecular Docking was used to study the binding of liliflorin A to dTAGGG(TTAGGG)3G-quadruplex HTG23. The CD data showed that liliflorin A enhanced HTG21 Tm. The Tm value of G-quadruplex was enhanced 3.2 ℃ by 4.0 μmol·L-1 liliflorin A in FRET. The NMR spectra of HTG21 showed vivid alteration after reacting with liliflorin A in 3 hours. Molecular Docking suggested liliflorin A bound to the wide groove of HTG23 at G9, G10, G16 and G17. Liliflorin A was the first lignan derivative that could stabilize HTG21 selectively and provided a new candidate for antitumor drug design targeting on human telomeric G-quadruplex.
刘婷婷1，周 爽1，贾千澜1，王文蜀1,2*，闫晓倩1，张文浩3，王帅旗1，焦玉国1 . 紫玉兰素A与人端粒G-四链体相互作用的光谱学研究，第一个木脂素类衍生物与G-四链体相互作用 [J]. 光谱学与光谱分析, 2016, 36(03): 896-902.
LIU Ting-ting1, ZHOU Shuang1, JIA Qian-lan1, WANG Wen-shu1,2*, YAN Xiao-qian1, ZHANG Wen-hao3, WANG Shuai-qi1, JIAO Yu-guo1. Spectral Analysis of Interaction between Human Telomeric G-Quadruplex and Liliflorin A, the First Lignan Derivative Interacted with G-Quadruplex DNA. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2016, 36(03): 896-902.
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