Abstract：In order to solve the problem of on-site rapid detection of salbutamol residues in feed and animal products, and develop a new method of fast detection of salbutamol on the basis of the molecular imprinting technology, this article uses the salbutamol (SAL) working as template molecule, methacrylic acid (MAA) working as functional monomer. On this basis, a new type of core-shell type salbutamol molecularly imprinted polymers were prepared with colloidal gold particles as triggering core. Superficial characteristics of the MIPs and the related compounds were investigated by ultraviolet (UV) spectra and infrared (IR) spectra, Raman spectra，Scanning electron microscopy （SEM） respectively. The results indicated that a stable hydrogen bonding complex has been formed between the carboxyl groups of SAL and MA with a matching ratio of 1∶1. The complex can be easily eluted by the reagent containing hydrogen bonding. The chemical binding constant K reaches -0.245×106 L2·mol-2. The possible binding sites of the hydrogen bonding was formed between the hydrogen atoms of —COOH in MA and the oxygen atoms of CO in SAL. IR and Raman spectrum showed that, compared with MA, a significant red shift of —OH absorption peak was manifested in MIPs, which proved that SAL as template molecule occurred a specific bond between MA. Red shift of stretching vibration absorption peak of CO was also detected in the un-eluted MIPs and obvious energy loss happened, which demonstrated a possible binding sites is SAL intramolecular of CO atom of oxygen. If the hydrogen atoms of —COOH in MA wanted to generate hydrogen bond. However, the shapes of absorption peak of other functional groups including CC， CO， and —OH were very similar both in MIPs and NIPs. Specific cavities were formed after the template molecules in MIPs were removed. It was proved by the adsorption experiment that the specific sites in these cavities highly match with the chemical and space structure of SAL. Besides，colloidal gold type core-shell molecularly imprinted polymers have looser surface, more cavities in the surface compared with ordinary molecularly imprinted polymers, which increased the effective area of adsorption to target molecules. So it have better performance in adsorption. Based on the principle that these cavities can specificly recognize and combine with target molecule in the test sample, and the excellent ability of colloidal gold core-shell molecularly imprinted polymers, the development of novel methods for fast determination of SAL based on the molecular imprinting technology can be expected in the near future.
Key words：Salbutamol;Core-shell molecularly imprinted polymers;Infrared spectra;Scanning electron microscopy;Rapid detection
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