ICP-MS法测定板栗自交与异交后子房微量元素的含量变化

doi:10.3964/j.issn.1000-0593(2015)10-2867-05

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摘要：为探明板栗授粉后子房的微量元素需求分配规律，应用电感耦合等离子体质谱测定与分析板栗自交与异交子房主要微量元素的含量。结果表明：板栗自交与异交5～50 d，六种微量元素均呈波动的变化趋势。授粉后20 d，异交Ca含量达到最高为6.50 mg·g^-1，而自交后10 d，子房的Ca含量达到最高为7.77 mg·g^-1。授粉后30 d，异交和自交子房Mg含量都达到最高，分别为4.19和4.69 mg·g^-1。授粉后5 d，异交子房Zn含量达到最高，为0.038 7 mg·g^-1，而自交后10 d达到最高为0.039 9 mg·g^-1。授粉后35 d，自交和异交子房Fe含量达到最高，分别为0.022和0.019 mg·g^-1。授粉后20 d，异交和自交子房Cu含量达到最高，分别为0.056和0.045 mg·g^-1。授粉后40 d，自交子房Mn含量达到最高，为1.204 mg·g^-1，而异交30 d，其子房Mn含量达到最高为0.845 mg·g^-1。研究结果可为在生产上喷施微肥来调控板栗子房发育提供参考依据，从而来提高板栗产量。

关键词：板栗;自交;异交;子房;微量元素

Abstract：Castanea mollissima Blume has potential as an non-wood forest trees that have been cultivated for thousands of years in China. In order to elucidate the trace elements of chestnut ovary, the major trace elements of self- and cross-pollination chestnut ovary were determined by inductively coupled plasma mass spectrometry. The results showed that self- and cross-pollination 5～50 d, six trace elements trends showed fluctuations. After cross-pollination 20 d, the content of Ca was up to 6.50 mg·g^-1, while the self-pollination 10 d, the content of Ca reached up to 7.77 mg·g^-1. After cross- and self-pollination pollination 30 d, the content of Mg were highest, 4.19 and 4.69 mg·g^-1, respectively. After cross-pollination 5 d, the content of Zn reached the highest, 0.038 7 mg·g^-1, while self-pollination 10d the content of Zn was 0.039 9 mg·g^-1. After self- and cross-pollination 35 d, the content of Fe were 0.022, 0.019 mg·g^-1, respectively. After cross- and self-pollination 20 d, the content of Cu were 0.056, 0.045 mg·g^-1, respectively. After self-pollination 40d, the content of Mn reaching the highest was 1.204 mg·g^-1, while cross-pollination 30 d, the content of Mn reached its maximum 0.845 mg·g^-1. The results can provide a reference for spraying fertilizer on the ovary development, thereby improving chestnut production.

Key words：Castanea mollissima;Self-pollination;Cross-pollination;ovary;Trace elements

收稿日期: 2014-06-06 修订日期: 2014-10-25

中图分类号:

O657.3

通讯作者: 郭素娟 E-mail: guosujuan2011@sina.com

引用本文:

邹锋，郭素娟^*，王静，彭晶晶，张小娜，彭娅琴 . ICP-MS法测定板栗自交与异交后子房微量元素的含量变化 [J]. 光谱学与光谱分析, 2015, 35(10): 2867-2871.
ZOU Feng, GUO Su-juan^*, WANG Jing, PENG Jing-jing, ZHANG Xiao-na, PENG Ya-qin . Determination the Change of Main Trace Elements in the Ovary with Self- and Cross-Pollination of Chinese Chestnut by ICP-MS. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2015, 35(10): 2867-2871.

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