Abstract：In the present paper the basic theory that organic molecules can emit fluorescence as they are excited by ultraviolet rays is described, the molecular structures of a few common pesticides, such as carbamate, benzoylurea and fungicide, are analyzed, and the mechanism of fluorescence generation is also ascertained. Consequently, the theoretic basis for further detection of pesticides by means of fluorescence methods is provided. Moreover a steady-state fluorescence spectrograph was applied to conduct fluorescence spectrum experiments with standard solutions of these pesticides, the fluorescence spectra were obtained, and their fluorescence characteristics were also analyzed. The results indicate that carbamate, benzoylurea and fungicide pesticides may emit strong fluorescence when excited by UV rays under the condition of a certain solvent, their fluorescence spectra are distinct, and the resolution is fine. As a result, it is feasible to carry out qualitative and the quantitative analysis of the concerned pesticides by fluorescence spectral analysis.
王忠东1,李东明1,王玉田2. 有机杀虫剂荧光光谱检测实验研究[J]. 光谱学与光谱分析, 2007, 27(11): 2321-2324.
WANG Zhong-dong1,LI Dong-ming1,WANG Yu-tian2. Study on the Detection of Organic Pesticides by Fluorescence Spectra. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2007, 27(11): 2321-2324.
[1] HUA Xiao-mei, JIANG Xi-liu(华小梅, 江希流). Environment Science Protection(环境保护), 2001, (6): 35. [2] National Pesticide Survey: Update and Summary of PhaseⅡ Results. Office of Water & Office of Pesticides and Toxic Substances, United States. US-EPA, 1999: 35. [3] XIA Jin-yao(夏锦尧). Practical Fluorescence Spectroscopic Analysis(实用荧光分析法). Beijing: Chinese People’s Public Security University Press(北京: 中国人民公安大学出版社), 1999. 46. [4] SU Yu, LIAO Xian-wei, LI Shu-wei, et al(苏 宇, 廖显威, 李树伟, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2003, 23(1): 137. [5] TANG Chu-chi, LI Yu-chang, CHEN Bin, et al(唐除痴, 李煜昶, 陈 彬, 等). Pesticide Chemistry(农药化学). Tianjin: Nankan University Press(天津：南开大学出版社), 2000. 140. [6] Digambara Patra, Mishra A K. Trends in Analytical Chemistry, 2002, 21(12): 787. [7] WANG Zhong-dong, WANG Yu-tian(王忠东, 王玉田). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2005, 25(10): 1645.
