Determination of Constituents of Polysaccharide and the Content of Saccharide in Mongolian Medicine Garidi-15
CUI Yi1,BAI Hai-quan2,ZHAO Yu-ying1*,Wulangerile1,SONG Juan-juan1,XU Xiu-ting1
1. Inner Mongolia Nationalities University, Tongliao 028043, China 2. Inner Mongolia KeYouZhongQi Teaching and Research Section, Xinganmeng 029000, China
Abstract：The present paper introuduces the method, with which the water-soluble polysaccharide was extracted with hot water from Garidi-15, the products were further purified with column chromatography on SephadexC-25, and the contents of water soluble polysaccharide were determined by phenyl hydrate-sulfuric acid method. The average recovery was 100.50%, and RSD was 0.82%. The components of water soluble polysaccharide were identified by gas chromatography as xylose, mannose, galactose and glucose in the molar ratio of 1.03∶1.26∶0.77∶2.30. The water soluble polysaccharide was identified by IR spectrum and UV(200-400 nm) scanning spectrum. The IR spectrum indicates that there are characteristic absorption peaks of polysaccharides at 3 417.46, 2 928.65, 1 742.86, 1 643.69, 1 149.78, 1 078.19, 1 022.56 and 834.57 cm-1, of which there are absorption peaks of pyranose at 1 078.19 and 1 022.56 cm-1,and contain one characteristic absorption peak of α-pyranose indican band at 834.57 cm-1. The UV scanning spectrum shows the obvious polysaccharide absorption peak at 280 nm, and indicates the existence of CO bond.
崔毅1,白海泉2,赵玉英1*,乌兰格日乐1,宋娟娟1,徐秀廷1 . 蒙药嘎日迪-15中多糖的研究[J]. 光谱学与光谱分析, 2007, 27(11): 2246-2248.
CUI Yi1,BAI Hai-quan2,ZHAO Yu-ying1*,Wulangerile1,SONG Juan-juan1,XU Xiu-ting1 . Determination of Constituents of Polysaccharide and the Content of Saccharide in Mongolian Medicine Garidi-15. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2007, 27(11): 2246-2248.
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