Biosorption of Radionuclide Uranium by Deinococcus Radiodurans
YANG Jie1, DONG Fa-qin1, 2*, DAI Qun-wei1, 2, LIU Ming-xue3, NIE Xiao-qin4, ZHANG Dong5, MA Jia-lin4, ZHOU Xian4
1. Environment and Resource College, Southwest University of Science and Technology, Mianyang 621010, China 2. Key Laboratory of Solid Waste Treatment and Resource Recycle, Ministry of Education, Mianyang 621010, China 3. Life Science and Engineering College, Southwest University of Science and Technology, Mianyang 621010, China 4. National Defence Science and Technology College, Southwest University of Science and Technology, Mianyang 621010, China 5. Institute of Nuclear Physics and Chemistry, China Academy of Engineering Physics, Mianyang 621900, China
Abstract：As a biological adsorbent, Living deinococcus radiodurans was used for removing radionuclide uranium in the aqueous solution. The effect factors on biosorption of radionuclide uranium were researched in the present paper, including solution pH values and initial uranium concentration. Meanwhile, the biosorption mechanism was researched by the method of FTIR and SEM/EDS. The results show that the optimum conditions for biosorption are as follows: pH=5, c0=100 mg·L-1 and the maximum biosorption capacity is up to 240 mgU·g-1. According to the SEM results and EDXS analysis, it is indicated that the cell surface is attached by lots of sheet uranium crystals, and the main biosorpiton way of uranium is the ion exchange or surface complexation. Comparing FTIR spectra and FTIR fitting spectra before and after biosorption, we can find that the whole spectra has a certain change, particularly active groups (such as amide groups of the protein, hydroxy, carboxyl and phosphate group) are involved in the biosorption process. Then, there is a new peak at 906 cm-1 and it is a stretching vibration peak of UO2+2. Obviously, it is possible that as an anti radiation microorganism, deinococcus radiodurans could be used for removing radionuclide uranium in radiation environment.
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