摘要： 旨在建立基于二维相关红外光谱(2D IR spectra)的分析技术，以进行中药中是否掺杂化学药物的判别。以减肥药盐酸芬氟拉明(fenfluramine hydrochloride, FH)、盐酸西布曲明(sibutramine hydrochloride, SH)为待检化合物，确定二者同步相关谱中的特征相关峰。首先通过比较掺杂中药与盐酸芬氟拉明的同步谱中特征相关峰的相似性，初步对掺杂化合物盐酸芬氟拉明进行判定，而后利用掺杂中药异步谱能确认相关峰来源的特性，进一步确证盐酸芬氟拉明的存在；此外，根据掺杂中药同步谱中未出现与盐酸西布曲明同步谱相似的特征相关峰，可以直接判别其中未掺杂盐酸西布曲明。实验所建立的方法无需样品分离，速度快，成本低，为快速简便地进行复杂体系中是否存在化学药物的判别提供了一种新的可能的方法。
关键词：二维相关红外光谱法;中药掺伪;判别分析
Abstract：A new method was established, based on infrared spectroscopy-two dimensional (2D) correlation analysis, for the discriminative analysis of adulteration in traditional Chinese medicines (TCM). Fenfluramine hydrochloride (FH) and sibutramine hydrochloride (SH) were taken as examples of synthetic drugs (adulterant), and the correlative peaks of their synchronous 2D correlation spectra were found. Then the characteristics of the synchronous 2D correlation spectrum of the suspected TCM were compared with those of FH. Since the correlative peaks in the synchronous spectrum of the suspected TCM coincide well with those of FH, a positive conclusion could be drawn after further investigation of the asynchronous spectra of TCM, which could provide the information about the source of correlative peaks. On the contrary, the dissimilarity of the synchronous spectra of SH and TCM directly implies that the suspected TCM is not adulterated with SH. The method can be used for a correct discrimination on whether the TCM is adulterated with the synthetic drugs, it does not rely on sample separation, and provides a new simple and cost-effective alternative to test the adulteration of TCM.
Key words：Two dimensional correlation infrared spectroscopy(2D-IR);Adulterated traditional Chinese medicine;Discriminative analysis
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