Analysis of Samples from Wastewater Treatment Plant and Receiving Waters Using EEM Fluorescence Spectroscopy
LI Wei-hua1, LIU Yi-xin1, WANG Wei3, SHENG Guo-ping2*, YU Han-qing2, SHUAI Lei1
1. School of Environment and Energy Engineering, Key Laboratory of Anhui Province of Water Pollution Control and Wastewater Reuse, Anhui Jianzhu University, Hefei 230601, China 2. Department of Chemistry, University of Science and Technology of China, Hefei 230026, China 3. School of Civil Engineering，Hefei University of Technology，Hefei 230009，China
Abstract：The samples from several treatment units in wastewater treatment plant (WWTP) and different sample sites of receiving waters were characterized using excitation-emission matrix (EEM) fluorescence spectroscopy. After analyzed by parallel factor analysis (PARAFAC) method, the EEM fluorescence spectra of the principal components and fluorescence intensity scores matrix were acquired. Results showed that protein-like and fulvic-like substances were the main components of the samples from the WWTP and the receiving water body. The intensity of protein-like fluorescence in the influent samples was rather strong, and those of the following samples decreased significantly. The protein-like fluorescence intensity of the samples was relatively weak from the upstream of receiving water body, while those of the samples increased significantly from the water body near the urban area. The protein-like fluorescence intensity of upstream samples of the WWTP disposal outlet was stronger than that of downstream in the receiving waters. The fluorescence intensity scores of protein-like substance could be correlated with the COD concentration of the samples and the correlation curves were established. The correlation coefficient of the WWTP samples was 0.930 and that of receiving water body samples was 0.913. The protein-like fluorescence could be used to evaluate the organic pollution of the samples. This study will provide a new method to investigate the operation status of WWTP and corresponding effect on the receiving water body.
[1] Molinos-Senante M, Garrido-Baserba M, Reif R, et al. Science of the Total Environment, 2012, 427-428: 11. [2] Hudson N, Baker A, Ward D, et al. Science of the Total Environment, 2008, 391: 149. [3] Coble P G. Marine Chemistry, 1996, 51：325. [4] Pons M N, Bonté S L, Potier O. Journal of Biotechnology, 2004, 113(1-3): 211. [5] Sheng G P, Yu H Q. Water Research, 2006, 40(6): 1233. [6] Li W H, Sheng G P, Liu X W, et al. Water Research, 2008, 42(12): 3173. [7] Wang Z W, Wu Z C, Tang S J. Water Research, 2009, 43(6): 1533. [8] GUO Wei-dong, YANG Li-yang, WANG Fu-li, et al(郭卫东，杨丽阳，王福利，等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2011, 31(2): 427. [9] Baker A, Inverarity R, Charlton M, et al. Environmental Pollution, 2003, 124: 57. [10] Baker A, Ward D, Lieten S H, et al. Water Research, 2004, 38: 2934. [11] CHEN Mao-fu, WU Jing, Lü Yan-li, et al(陈茂福，吴 静，律严励，等). Acta Optica Sinica(光学学报)，2008, 28(3): 578. [12] WANG Zhi-gang, LIU Wen-qing, LI Hong-bin, et al(王志刚，刘文清，李宏斌，等). Journal of Environmental Science(环境科学学报), 2006, 26(2): 275. [13] Zhang Y L, Yin Y, Feng L Q, et al. Water Research, 2011, 45(16): 5110. [14] Bro R. Chemometrics and Intelligent Laboratory Systems, 1997, 38(2): 149. [15] Bro R, Kiers H A L. Journal of Chemometrics, 2003, 17: 274. [16] Cohen E, Levy G J, Borisover M. Water Research, 2014, 55: 323. [17] Farabegoli G, Hellinga C, Heijnen J J, et al. Water Research, 2003, 37: 2732.
