1. Department of Physics, Xuzhou Institute of Industry Technology, Xuzhou 221006, China 2. School of Science, Nanjing University of Science and Technology, Nanjing 210094, China 3. The Third People’s Hospital of Xuzhou, Xuzhou 221005, China
Abstract:Protoporphyrin IX is an important kind of organic compound for vital movement, and can be used as the sign of tumour blood. Human protoporphyrin IX content in serum is very low, and affected by various factors. The serum fluorescence spectrum analysis system based on wavelet transform was used to discriminated the protoporphyrin IX weak signals. The protoporphyrin IX fluorescence spectrum was obtained by a multi-function spectrum measuring system, and decomposed several times by wavelet transform to distinguish the noise and spectrum signals. The fluorescence spectrum can be divided into corresponding discrete approximations signals (a1-a6) and discrete details signals (d1-d6) by six times of decomposition, showing the signal frequency decreasing with decomposition times increasing and the protoporphyrin IX fluorescence character peak appears here. The weak signals were discriminated and the exactly component and quantity can be acquired for further analysis. So it can be analysed quantitatively. The researches in the present paper provide the potential application in the diagnosis of incipient tumous using the serum fluorescence spectrum.
[1] LIN San-ren, LI Cheng-gang, ZHOU Li-ya, et al(林三仁,李成刚,周丽雅,等). J. of Beijing Medical University(北京医科大学学报), 1997, 29(4): 341. [2] Masilamani V, Al-Zhrani K, Al-Salhi M, et al. J. of Luminescence, 2004, 109: 143. [3] Kojiro Onizawa, Naomichi Okamura, Hideo Saginoyac. Oral Oncology, 2002, 38(4): 343. [4] MENG Ji-wu, Nishisaka T, Fukami T. Chin. Sci. Bull., 1995, 40: 1144. [5] MENG Ji-wu, HOU Shang-gong, XU Xu-rong(孟继武,侯尚公,徐叙). Chinese Science Bulletin(科学通报), 1988, 33: 630. [6] Meng J W, Wang X J, Lin T, et al. J. of Luminescence, 1999, 83-84: 271. [7] MENG Ji-wu, GU Huai-min, ZHENG Rong-er, et al(孟继武,谷怀民,郑荣儿,等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2003, 23(2): 325. [8] LIAN Shao-hui, YANG Shi-zhen, WANG Jia-ping(连少辉, 杨士珍,王家平). Bulletin of National Natural Science Foundation of China(中国科学基金), 1997, 11: 288. [9] Michael Vogeser, Karl Jacob, Reinhart Zachoval. Clinical Biochemistry, 2000, 33(5): 387. [10] LI Shao-hua, HAO Ying-hua, GAO Shan-ling, et al(李少华, 郝英华, 高善玲, 等). Chinese Journal of Oncology(中华肿瘤杂志), 1996, 18(1): 34. [11] QIN Xia, SHEN Lan-sun(秦 侠,沈兰荪). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2000, 20(6): 892. [12] WANG Shu-tao, WANG Yu-tian, CHE Ren-sheng, et al(王书涛,王玉田,车仁生,等). J. Applied Optics(应用光学), 2005, 26(1): 49. [13] WANG Ying, MO Jin-yuan(王 瑛,莫金垣). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2005, 25(1): 124. [14] CHEN Li-na, GAO Zhi-wen, LI Kong-ning(陈丽娜,高治文,李孔宁). J. of Harbing Medical University(哈尔滨医科大学学报), 1995, 29(1): 1.