Determination of Terbium(Ⅲ) with EHPG-Tb(Ⅲ) System by Fluorescence Spectroscopy
ZHAO Chun-gui,LI Xiao-li,YANG Bin-sheng
Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Biotechnology, Shanxi University, Taiyuan 030006, China
Abstract：The fluorescence of terbium was sensitized after addition of terbium to the ethylene-N, N’-bis (o-hydioxyphenylglycine) (EHPG) solution. A novel and simple method used for the determination of Tb(Ⅲ) was developed by means of fluorescence spectroscopy in the presence of EHPG. It was showed that the relative fluorescence intensity is proportional to the concentration of terbium ions, while the molar ratio of terbium to EHPG is less than 1.0 in the system. The maximum wavelengths of excitation and emission are 295 and 547 nm respectively. The optimal range of pH is 7-9. The linear range of detection of the concentration of terbium is from 1.0×10-8mol·L-1 to 1.0×10-5 mol·L-1,with a detection limit of 1.18×10-9 mol·L-1. The relative standard deviation is still within ±3% in the presence of other lanthanide ions. The method was applied to the determination of the recoveries of synthetic samples and a rare earth sample with satisfactory results.
[1] NIE Li-hua, ZHAO Hui-chun, WANG Xu(聂丽华，赵慧春，王 旭). Chinese Journal of Analytical Chemistry(分析化学), 2001, 29: 910. [2] TONG Shang-lun, XIANG Guang-hong, CHEN Ping-ping, et al(童裳伦, 项光宏, 陈萍萍, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2004，24(12): 1612. [3] HE Hua, YE Hai-ying, DAI Li, et al(何 华, 叶海英, 戴 丽, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2006, 26(3): 480. [4] LUO Jian-ping, KANG Jin-wen, YANG Rui(罗建平, 亢锦文, 杨 锐). Metallurgical Standardization and Quality(冶金标准化与质量), 1998, 12: 6. [5] LIU Xiang-sheng, CAI Shao-qin, LIU Feng-fen(刘湘生, 蔡绍勤, 柳风粉). Chinese Journal of Analytical Chemistry(分析化学), 1997, 25(4): 431. [6] Jimidar M, Hamoir P, Degezelle W, et al. Anal. Chim. Acta, 1993, 284(1): 217. [7] WANG Xiao-li, ZHAO Hui-chun, LI Xia, et al(王晓丽, 赵慧春, 李 夏, 等). Chinese Journal of Analytical Chemistry(分析化学), 2005, 33: 647. [8] Brittain H G, River S. Inorg. Chim. Acta, 1985, 110(1): 35. [9] XU Shu-dao(许书道). Chinese Journal of Analytical Chemistry(分析化学), 2002, 30(10): 1257. [10] Xu Y Y, Hemmil Ilkka A. Talanta, 1992, 39(7): 759. [11] LI Ying-qi, BAI Hai-jing, YANG Bin-sheng(李英奇, 白海静, 杨斌盛). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2002, 22(3): 433. [12] Vincent L P, Wesley R H, Carl J C, et al. Biochemistry, 1981, 20: 7033. [13] YANG Bin-sheng(杨斌盛). Chinese Journal of Biochemistry and Molecular Biology(中国生物化学与分子生物学报), 1999, 15(3): 462. [14] MA Wen-yuan, YANG Jing-he, GE Hong-mei, et al(马文元, 杨景和, 葛红梅, 等). Physical Testing and Chemical Analysis Part B Chemical Analysis(理化检验: 化学分册), 1997, 33(6): 260.
