Determination of Photosynthetic Pigments in Citrus Leaves Based on Hyperspectral Images Datas
TIAN Xi1, 2, HE Shao-lan2, 3, Lü Qiang2, 3, YI Shi-lai2, 3, XIE Rang-jin2, 3, ZHENG Yong-qiang2, 3, LIAO Qiu-hong1, 2, DENG Lie2, 3*
1. College of Horticulture and Landscape Architecture,Southwest University,Chongqing 400715,China 2. Citrus Research Institute, Southwest University-Chinese Academy of Agricultural Sciences,Chongqing 400712,China 3. National Engineering Technology Research Center for Citrus,Chongqing 400712,China
Abstract:The effective region was segmented from the hyperspectral image of citrus leaf by threshold method with the average spectrum extracted and used to describe the corresponding leaf. Based on the different spectral pre-processing methods, the prediction models of three photosynthetic pigments (i.e., chlorophyll a, chlorophyll b, and carotenoid) were calibrated by partial least squares (PLS), BP neural network (BPNN) and least square support vector machine (LS-SVM). The LS-SVM model for chlorophyll a was established based on multiplicative scatter correction (MSC), and the correlation coefficient (Rp) and the root mean square error of prediction (RMSEP) were 0.898 3 and 0.140 4, respectively. The LS-SVM model for chlorophyll b with Rp=0.912 3 and RMSEP=0.042 6, was established based on standard normal variable (SNV). The PLS model for carotenoid was established with Rp=0.712 8 and RMSEP=0.062 4 based on moving average smoothing (MAS), but the result was no better than the other two. The results illustrated that these three photosynthetic pigments could be nondestructively and real time estimated by hyperspectral image.
[1] GUO Chun-ai,LIU Fang,XU Xiao-ming(郭春爱,刘 芳,许晓明). Plant Physiology Communications(植物生理学通讯),2006,42(5):967. [2] TANG Yan-lin,WANG Ji-hua,HUANG Jing-feng,et al(唐延林,王纪华,黄敬峰,等). Transactions of the Chinese Society of Agricultural Engineering(农业工程学报),2003,19(6):167. [3] Zarco-Tejada P J,Miller J R,Morales A,et al. Remote Sensing of Environment,2004,90(4):466. [4] LIU Yan-de,ZHANG Guang-wei,CAI Li-jun(刘燕德,张光伟,蔡丽君). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2012,32(12):3377. [5] Zou Xiaobo,Shi Jiyong,Hao Limin,et al. Analytica Chimica Acta,2011, 706(1),105. [6] ZHU Xi-cun,ZHAO Geng-xing,WANG Rui-yan,et al(朱西存,赵庚星,王瑞燕,等). Scientia Agricultura Sinica(中国农业科学),2010,43(6):1189. [7] LIU Xuan,LIN Hui,ZANG Zhuo,et al(刘 璇,林 辉,臧 卓,等). Journal of Central South University of Forestry & Technology(中南林业科技大学学报),2010,30(5):72. [8] SONG Kai-shan,ZHANG Bai,WANG Zong-ming,et al(宋开山,张 柏,王宗明,等). Chinese Journal of Plant Ecology(植物生态学报),2008,32(1):152. [9] YANG Jie,TIAN Yong-chao,YAO Xia,et al(杨 杰,田永超,姚 霞,等). Chinese Journal of Plant Ecology(植物生态学报),2010,34(7):845. [10] TANG Yan-lin,HUANG Jing-feng,WANG Xiu-zhen,et al(唐延林,黄敬峰,王秀珍,等). Journal of Maize Sciences(玉米科学),2008,16(2):71. [11] HUANG Shuang-ping,HONG Tian-sheng,YUE Xue-jun,et al(黄双萍,洪添胜,岳学军,等). Transactions of the Chinese Society of Agricultural Engineering(农业工程学报),2013,29(5):132. [12] HU Xian-ning(胡先宁). Science Mosaic(科技广场),2011,3:106. [13] HAN Lei,LI Rui,ZHU Hui-li(韩 磊,李 锐,朱会利). Transactions of the Chinese Society for Agricultural Machinery(农业机械学报),2011,42(7):109. [14] Vapnik V. IEEE Transactions on Neural Networks,1999,10(5):988. [15] YAN Wei-wu,SHAO Hui-he(阎威武,邵惠鹤). Control and Decision(控制与决策),2003,18(3):358. [16] WANG Wei,PENG Yan-kun,WANG Xiu,et al(王 伟,彭彦昆,王 秀,等). Journal of Agricultural Mechanization Research(农机化研究),2010,9:170. [17] LUAN Wei-lei,MENG Qing-jin,SHEN Tao(栾维磊,孟庆金,申 涛). Journal of University of Jinan Sci. & Tech.(济南大学学报·自然科学版),2013,27(4):347. [18] Suykens J A K,Vandew Alle J. Neural Network Letters,1999,9(3):294. [19] Suykens J A K,Vandew Alle J. IEEE Transactions on Circuits and System I,2000,47(7):1109. [20] WU Su-hua(武素华). Journal of Anhui Agri. Sci.(安徽农业科学),2009,37(9):3865. [21] NI Zhen,HU Chang-qin,FENG Fang(尼 珍,胡昌勤,冯 芳). Chinese Journal of Pharmaceutical Analysis(药物分析杂志),2008,28(5):824. [22] ZHANG Shu-juan,WANG Feng-hua,ZHANG Hai-hong,et al(张淑娟,王凤花,张海红,等). Journa of Shanxi Agricultural University(山西农业大学学报),2009,29(6):2579. [23] WANG Qiang,TIAN Xue-min(王 强,田学民). Journal of Beijing Institute of Technology(北京理工大学学报),2012,32(10):1031.