1. Hubei Key Laboratory of Bioanalytical Technology, Hubei Normal University, Huangshi 435002, China 2. Department of Chemistry, Central China Normal University, Wuhan 430079, China
Abstract：The inclusion of β-cyclodextrin (CD) for thionine (TH) and the interaction of DNA with CD-TH inclusion complex were investigated by fluorescence and visible absorption spectrometry. TH with β-CD formed a 1∶1 inclusion complex with the stability constant of 527 L·mol-1 (visible spectrometry)/444 L·mol-1 (fluorescence) in the pH 7.2 PBS buffer solutions. The addition of DNA makes the absorbance of the inclusion complex decrease and the absorption spectrum shift toward long wavelengths. The fluorescence experiments indicated that the presence of DNA makes the emission peak of CD-TH shift toward short wavelengths and the fluorescence of inclusion complex quench, and the quenching constant was calculated to be 6.12×104 L·mol-1 by Stern-Volmer method. All the data confirmed that CD-TH reacted with DNA in intercalative mode, and the binding numbers and the binding constant were estimated to be 1 and 3.47×104 L·mol-1 by spectrophotometry.
[1] Oliveira-Brett A M, Diculescu V C. Bioelectrochemistry, 2004, 64: 143. [2] Zhang N, Zhang X L, Zhao Y F. Talanta, 2004, 62: 1041. [3] DU Jiang-yan, HUANG Xiao-hua, XU Fei, et al(杜江燕, 黄晓华, 徐 飞, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2005, 25(9): 1435. [4] YE Xian-bao, LIU Ling-fang, QI Xiao-hua, et al(冶献保, 刘灵芳, 齐小花, 等). Journal of Analytical Science(分析科学学报), 2003, 19(1): 51. [5] DU Jiang-yan, HUANG Xiao-hua, XU Fei, et al(杜江燕, 黄晓华, 徐 飞, 等). Acta Phys. Chim. Sin.(物理化学学报), 2003, 19(11): 1064. [6] Rickus J L, Chang P L, Tobin A J, et al. J. Phys. Chem. B, 2004, 108: 9325. [7] Long X F, Tao X C, Wang Y Z, et al. Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy, 2004, 60(1-2): 455. [8] Xu Y, Yang L, Ye X Y, et al. Electroanalysis, 2006,18(9): 873. [9] Benesi H A, Hildebrand J H. J. Am. Chem. Soc., 1949, 17: 2703. [10] Chou J, Qu X G, Lu T H, et al. J. Microchem., 1995, 2: 159. [11] Pyle A M, Rehmamn J P, Meshoyrer R. J. Am. Chem. Soc., 1989, 111(8): 3051. [12] Kumar C V, Asuncion E H. J. Am. Chem. Soc., 1993, 115(19): 8547. [13] CHEN Guo-zhen, HUANG Xian-zhi, ZHENG Zhu-zi, et al(陈国珍, 黄贤智, 郑朱梓). Fluorescence Analysis(Second Edition)(荧光分析法, 第2版). Beijing: Science Press(北京: 科学出版社), 1990. 112. [14] Ware W R. J. Phys. Chem. 1962, 66: 455. [15] Gavin D R, Douglas J W, Mark A D. J. Am. Chem. Soc., 2001, 123: 6953. [16] Long E C, Barton J K. Accounts of Chemical Research, 1990, 23: 271. [17] CAO Ying, HE Xi-wen(曹 瑛, 何锡文). Chinese J. Anal. Chem.(分析化学), 1998, 26(10): 1165. [18] BIAN Wei, WEI Yu-xia, WEI Yan-li, et al(卞 伟, 魏玉霞, 魏艳丽, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2006, 26(1): 125.
