1. College of Science, Nanjing University of Aeronautics and Astronautics, Nanjing 210016, China 2. College of Science, Hohai University, Nanjing 210098, China
Abstract：Blood plays an important role in the clinical diagnosis and treatment; many diseases may lead to changes in the blood composition or characteristic properties. Therefore, the analysis of blood sugar will be of great importance. The present paper studies the difference of fluorescence intensity stimulated by light wave between normal blood serum and hyperglycemia blood serum. The result shows that the average grey level of fluorescence image at 365 nm increases obviously with the changes in the concentration of glucose; however, the average grey changes little at 353, 369 and 405 nm. This shows that the main effect of serum glucose on the fluorescence intensity peak appears at 365 nm. Because the fluorescence intensity has something to do with the concentration of the fluorescence material, the blood glucose concentration influences directly the fluorescence intensity at 365 nm. Therefore, it is possible to judge blood sugar content, and to make sure whether the content exceeds the normal value or not by comparing the fluorescence intensity of the blood serum measured at 365 nm. This technique of optical measurement provides an experimental basis for the diagnosis of blood diseases.
[1] YU Chang-qing，LIU Fan-guang，GU Ying，et al(于常青，刘凡光，顾 瑛，等). Chinese Journal of Laser Medicine & Surgery(中国激光医学杂志)，2004，13(1)：41. [2] DING Xiao-ping, MENG Chao, WANG Jian-lin, et al(丁小平，孟 超，王建林，等). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，1999，19(2)：225. [3] LIN Shang-zhong，CHEN Rong，XU Shao-feng(林上忠，陈 荣，许少锋). Chinese Journal of Physical Medicine(中华物理医学杂志)，1998，20(1)：26. [4] WANG Le-xin，ZHAO Zhi-min，YAO Hong-bing，et al(王乐新，赵志敏，姚红兵，等). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2002，22(6)：980. [5] XIE Ji-yun，JIANG Zhi-liang，ZHONG Fu-xin，et al(谢济运，蒋治良，钟福新，等). Analysis and Testing Technology and Instruments(分析测试技术与仪器)，2000，6(3)：178. [6] CHEN Rong, LI Bu-hong, CHEN Jin-kai, et al(陈 荣，李步洪，陈金铠，等). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2003，23(4)：748. [7] LI Jian-dong, DING Jian-hua, WU Min, et al(李建东，丁建华，吴 敏). Applied Laser(应用激光)，2004，24(2)：112. [8] ZHAO Zhi-min，CHEN Yu-ming，YU Xiao-lei，et al(赵志敏，陈玉明，俞晓磊，等). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2003，23(5)：922. [9] LAN Xiu-feng, LIU Jian-gang, LIU Ying, et al(兰秀风，刘建刚，刘 莹，等). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2006，26(13)：467. [10] ZHAO Zhi-min, CHEN Yu-ming, YU Xiao-lei(赵志敏，陈玉明，俞晓磊). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2003，23(5)：992. [11] CHEN Rong, CHEN Yan-jiao, YANG Wen-qin, et al(陈 荣，陈艳娇，杨文琴，等). Acta Laser Biology Sinica(激光生物学报)，1999，8(1)：62. [12] GE Xiang-hong, ZHAO Yuan-li, ZHANG Feng-qiu, et al(葛向红，赵元黎，张凤秋, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2004，24(7)：841.
