Preparation, Characterization and Surface-Enhanced Raman Properties of Agarose Gel/Gold Nanoparticles Hybrid
MA Xiao-yuan1, 2, 3, LIU Ying1, WANG Zhou-ping1, 3*
1. School of Food Science and Technology, Jiangnan University, Wuxi 214122, China 2. The State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096, China 3. Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi 214122, China
Abstract：Agarose gel/gold nanoparticles hybrid was prepared by adding gold nanoparticles to preformed agarose gel. Nanocomposite structures and properties were characterized by transmission electron microscopy (TEM)，scanning electron microscopy(SEM)、and UV-Vis-NIR absorption spectroscopy. Experimental data indicated a uniform distribution of gold nanoparticles adsorbed on agarose gel network. And the excellent optical absorption properties were shown. Based on the swelling-contraction characteristics of agarose gel and the adjustable localized surface plasmon resonance (LSPR) of the gold nanoparticles, the nanocomposites were used as surface enhanced Raman scattering (SERS) substrate to detect the Raman signal molecules Nile blue A. Results revealed that the porous structure of the agarose gel provided a good carrier for the enrichment of the gold nanoparticles. The gold nanoparticles dynamic hot-spot effect arising from the agarose gel contraction loss of water in the air greatly enhanced the Raman signal.
[1] Daniel M C, Astruc D. Chem. Rev., 2004, 104: 293. [2] Aldeanueva-Potel P, Faoucher E, Alvarez-Puebla R A, et al. Anal. Chem., 2009, 81: 9233. [3] Jakob M, Levanon H, Kamat P V. Nano Lett., 2003, 3: 353. [4] Li X Z, Li F B, Environ. Sci. Technol., 2001, 35: 2381. [5] Fatin-Rouge N, Milon A, Buffle J, et al. J. Phys. Chem. B, 2003, 107: 12126. [6] Murano E. J. Appl. Phycol., 1995, 7: 245. [7] Golightly R S, Doering W E, Natan M J. ACS Nano, 2009, 3: 2859. [8] Lin M, He L, Awika J, et al. J. Food Sci., 2008, 73: T129. [9] Hering K, Cialla D, Ackermann K, et al. Anal. Bioanal. Chem., 2007, 390: 113. [10] Pieczonka N P W, Aroca R F. Chem. Phys. Chem., 2005, 6: 2473. [11] Porter M D, Lipert R J, Siperko L M, et al. Chem. Soc. Rev., 2008, 37: 1001. [12] Phadtare S, Kumar A, Vinod V P, et al. Chem. Mater., 2003, 15: 1944. [13] Liz-Marzan L M. Langmuir, 2006, 22: 32. [14] Yoo H, Millstone J E, Li S Z, et al. Nano Lett., 2009, 9: 3038. [15] Lawless M K, Mathies R A. J. Chem. Phys., 1992, 96: 8037. [16] Nascimento G M, Temperini M L A. J. Raman Spectrosc., 2008, 39: 772. [17] Aldeanueva-Potel P, Faoucher E, Alvarez-Puebla R A, et al. Anal. Chem., 2009, 81: 9233. [18] Olivier R B, Jean-Francois M. Anal. Chem., 2011, 83: 8057.
