Study on Panax Notoginseng and Its Processed Products by FTIR Spectroscopy
HUANG Dong-lan1, CHEN Xiao-kang1*, XU Yong-qun1, SUN Su-qin2, LU Wen-guan1
1. School of Chemistry and Environmental Engineering, Shaoguan University, Shaoguan 512005, China 2. Department of Chemistry, Tsinghua University, Beijing 100084, China
Abstract:The chemical differences of panax notoginseng before and after processing were analyzed by Fourier transform Infrared spectroscopy (FTIR) combined with two-dimensionalcorrelation spectroscopy (2D-IR). Compared with conventional IR spectra of the samples, the FTIR spectra of panax notoginseng and its processed products were similar in the range of 1 200~400 cm-1. The difference was that prepared panax notoginseng had strong and characteristic peaks at 2 925,2 855,1 746,1 460,1 376 and 1 158 cm-1, which all arose from the characteristic vibration of peanut oil. This was because there was some peanut oil left in the panax notoginseng, when panax notoginseng after processing. Obvious differences were observed between 2D-IR spectra of them, in the range of 1 400~1 700 cm-1, there was only one auto peaks at 1 650 cm-1 in the spectra of panax notoginseng, but there were auto peaks at 1 469 and 1 640 cm-1 in the spectra of prepared panax notoginseng. In the range of 1 400~1 700 cm-1, the 2D-IR spectra of panax notoginseng and its processed product present characterstic peaks at 1 139(1 137),1 194(1 196),1 121(1 221)cm-1 respectively, but the relative intensities of auto peaks were changed. For example, auto peak around 1 139 cm-1 was enhanced, but auto peak around 1 194 cm-1 was weakened. The results of 2D-IR correlation spectroscopy indicated the decomposition of flavonoids, saccharides and saponins. This method can track dynamically the processingprocedure of panax notoginseng and reveal the main tansformations, so it can explain the pharmacology of panax notoginseng and its processed product by FTIR and 2D-IR.
[1] Pharmacopoeia Committee of Ministry of Health, the People’s Republic of China(中华人民共和国药典委员会). Pharmacopeia of the People’s Republic of China·Ⅰ(中华人民共和国药典·一部). Beijing: Chemical Industry Press(北京:化学工业出版社), 2010. [2] Wang D, Liao P Y, Zhu H T, et al. Food Chemistry, 2012, 132: 1808. [3] Lau A J, Toh D F, Chua T K, et al. Journal of Ethnopharmacology, 2009, 125: 380. [4] GUO Lei, SHI Lei(郭 雷, 石 磊). Modern Journal of Integrated Traditional Chinese and Western Medicine(现代中西医结合杂志), 2010, 19(10): 1290. [5] CHEN Bin, XU Hui-lin, JIA Xiao-bin(陈 斌, 许慧琳, 贾晓斌). Chinese Traditional and Herbal Drugs(中草药), 2013, 44(4): 482. [6] Li D, Jin Z X, Zhou Q, et al. Journal of Molecular Structure, 2010, 974: 68. [7] LIU Su-li, CHEN Jian-bo, ZHOU Qun, et al(刘素丽, 陈建波, 周 群, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2012, 32(10): 2669. [8] Choong Y K, SUN S Q,Zhou Q, et al. Vibrational Spectroscopy, 2011, 57: 87. [9] Zhou Q, Chen J B, Sun S Q. Biomemdical Spectroscopy and Imaging, 2013, 2: 101. [10] Guangxi Food and Drug Administration(广西壮族自治区食品药品监督管理局). Chinese Herbal Medicine Preparation Standards of Guangxi(广西壮族自治区中药饮片炮制规范). Nanning: Guangxi Science and Technology Press(南宁:广西科学技术出版社), 2007. [11] Sun S, Wang C Z, Tong R, et al. Food Chemistry, 2010, 118: 307.