Revealing the Chemical Changes of Tea Cell Wall Induced by Anthracnose with Confocal Raman Microscopy
LI Xiao-li1, LUO Liu-bin1, HU Xiao-qian2, LOU Bing-gan3, HE Yong1*
1. College of Biosystems Engineering and Food Science，Zhejiang University，Hangzhou 310058，China 2. Agricultural Experiment Station, Zhejiang University，Hangzhou 310058，China 3. Institute of Biotechnology, Zhejiang University, Hangzhou 310058，China
Abstract：Healthy tea and tea infected by anthracnose were first studied by confocal Raman microscopy to illustrate chemical changes of cell wall in the present paper. Firstly, Raman spectra of both healthy and infected sample tissues were collected with spatial resolution at micron-level, and ultrastructure of healthy and infected tea cells was got from scanning electron microscope. These results showed that there were significant changes in Raman shift and Raman intensity between healthy and infected cell walls, indicating that great differences occurred in chemical compositions of cell walls between healthy and infected samples. In details, intensities at many Raman bands which were closely associated with cellulose, pectin, esters were reduced after infection, revealing that the content of chemical compounds such as cellulose, pectin, esters was decreased after infection. Subsequently, chemical imaging of both healthy and infected tea cell walls were realized based on Raman fingerprint spectra of cellulose and microscopic spatial structure. It was found that not only the content of cellulose was reduced greatly after infection, but also the ordered structure of cellulose was destroyed by anthracnose infection. Thus, confocal Raman microscopy was shown to be a powerful tool to detect the chemical changes in cell wall of tea caused by anthracnose without any chemical treatment or staining. This research firstly applied confocal Raman microscopy in phytopathology for the study of interactive relationship between host and pathogen, and it will also open a new way for intensive study of host-pathogen at cellular level.
李晓丽1，罗榴彬1，胡小倩2，楼兵干3，何 勇1* . 基于共聚焦显微拉曼光谱揭示炭疽病侵染下茶叶细胞壁变化的研究 [J]. 光谱学与光谱分析, 2014, 34(06): 1571-1576.
LI Xiao-li1, LUO Liu-bin1, HU Xiao-qian2, LOU Bing-gan3, HE Yong1* . Revealing the Chemical Changes of Tea Cell Wall Induced by Anthracnose with Confocal Raman Microscopy . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2014, 34(06): 1571-1576.
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