Analysis of Poly-β-Hydroxyalkonates (PHA) During the Enhanced Biological Phosphorus Removal Process Using FTIR Spectroscopy
LI Wei-hua1,2, MAO Qin-yan1, LIU Yi-xin1, SHENG Guo-ping2*, YU Han-qing2, HUANG Xian-huai1, LIU Shao-geng1, LING Qi1, YAN Guo-bing1
1. School of Environment and Energy Engineering, Key Laboratory of Anhui Province of Water Pollution Control and Wastewater Reuse, Anhui Jianzhu University, Hefei 230601, China 2. Department of Chemistry, University of Science and Technology of China, Hefei 230026, China
Abstract：Enhanced biological phosphorus removal (EBPR) is the main phosphorus removal technique for wastewater treatment. During the anaerobic-aerobic alternative process, the activated sludge experienced the anaerobic storage of polyhydroxy-β-alkonates (PHA) and aerobic degradation, corresponding the infrared peak intensity of sludge at 1 740 cm-1 increased in the aerobic phase and declined in the anaerobic phase. Compared with PHA standard, this peak was indentified to attribute the carbonyl of PHA. The overlapping peaks of PHA, protein Ⅰ and Ⅱ bands were separated using Gaussian peak fitting method. The infrared peak area ratios of PHA versus protein I had a good relationship with the PHA contents measured by gas chromatography, and the correlation coefficient was 0.873. Thus, the ratio of the peak area of PHA versus protein Ⅰ can be considered as the indicator of the PHA content in the sludge. The infrared spectra of 1 480~1 780 cm-1 was selected, normalized and transferred to the absorption data. Combined with the chromatography analysis of PHA content in the sludge sample, a model between the Fourier-transform infrared spectroscopy (FTIR) spectra of the sludge and PHA content was established, which could be used for the prediction of the PHA content in the unknown sample. The PHA content in the sludge sample could be acquired by the infrared spectra of the sludge sample and the established model, and the values fitted well with the results obtained from chromatograph. The results would provide a novel analysis method for the rapid characterization and quantitative determination of the intracellular PHA content in the activated sludge.
Key words：Infrared spectra;Poly-β-hydroxyalkonate;Gaussian peak fitting;Partial least squares
李卫华1,2，毛钦焱1，刘怡心1，盛国平2*，俞汉青2，黄显怀1，刘绍根1，凌 琪1，严国兵1 . 强化生物除磷过程中聚β羟基烷酸酯的红外光谱分析 [J]. 光谱学与光谱分析, 2014, 34(06): 1512-1517.
LI Wei-hua1,2, MAO Qin-yan1, LIU Yi-xin1, SHENG Guo-ping2*, YU Han-qing2, HUANG Xian-huai1, LIU Shao-geng1, LING Qi1, YAN Guo-bing1. Analysis of Poly-β-Hydroxyalkonates (PHA) During the Enhanced Biological Phosphorus Removal Process Using FTIR Spectroscopy. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2014, 34(06): 1512-1517.
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