Rapid Detection of Four Antipertensive Chemicals Adulterated in Traditional Chinese Medicine for Hypertension Using TLC-SERS
ZHU Qing-xia1, 2, CAO Yong-bing1, 2, CAO Ying-ying1, 2, LU Feng1, 2*
1. School of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350108, China 2. School of Pharmacy, Second Military Medical University, Shanghai 200433, China
Abstract：A novel facile method for on-site detection of antipertensive chemicals (e.g. nicardipine hydrochloride, doxazosin mesylate, propranolol hydrochloride, and hydrochlorothiazide) adulterated in traditional Chinese medicine for hypertension using thin layer chromatography (TLC) combined with surface enhanced Raman spectroscopy (SERS) was reported in the present paper. Analytes and pharmaceutical matrices was separated by TLC, then SERS method was used to complete qualitative identification of trace substances on TLC plate. By optimizing colloidal silver concentration and developing solvent, as well as exploring the optimal limits of detection (LOD), the initially established TLC-SERS method was used to detect real hypertension Chinese pharmaceuticals. The results showed that this method had good specificity for the four chemicals and high sensitivity with a limit of detection as lower as to 0.005 μg. Finally, two of the ten antipertensive drugs were detected to be adulterated with chemicals. This simple and fast method can realize rapid detection of chemicals illegally for doping in antipertensive Chinese pharmaceuticals，and would have good prospects in on-site detection of chemicals for doping in Chinese pharmaceuticals.
Key words：TLC;SERS;Traditional Chinese medicine;Adulteration
朱青霞1, 2，曹永兵1, 2，曹颖瑛1, 2，陆 峰1, 2* . TLC-SERS法快速检测降压类中药中非法添加的四种化学成分 [J]. 光谱学与光谱分析, 2014, 34(04): 990-993.
ZHU Qing-xia1, 2, CAO Yong-bing1, 2, CAO Ying-ying1, 2, LU Feng1, 2* . Rapid Detection of Four Antipertensive Chemicals Adulterated in Traditional Chinese Medicine for Hypertension Using TLC-SERS. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2014, 34(04): 990-993.
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