Nondestructive Recognization of Mountain Cultivated Ginseng and Garden Cultivated Ginseng by FTIR Microspectroscopy
BU Hai-bo1, WANG Feng2, LIN Hong-ying1, GUO Zhi-yong1, YUAN Shao-xiong1, PAN Li-li1，XU Xiao-jia1, LI Xiang-ri1*, WANG Gang-li2*, LIN Rui-chao1, 2
1. School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China 2. National Institutes for Food and Drug Control, State Food and Drug Administration, Beijing 100050, China
Abstract：Fourier transform infrared (FTIR) microspectroscopy technology is the combination of the FTIR spectrometer and the microscope. This technology is of simple preparation of the samples, can be used in micro-area analysis and micro-samples, and reflect the nature of the samples spectra. Panax ginseng include mountain cultivated ginseng (MCG), garden cultivated ginseng (GCG) and mountain wild ginseng (MWG), but the excavation of MWG is prohibited in China. So, only MCG and GCG were collected and recorded in Chinese pharmacopoeia. In this study, we developed a discriminant analysis (DA) method for recognition of MCG and GCG using FTIR microspectroscopy technology. Twenty MCG samples and twenty four GCG samples were obtained, and their spectra of IR microspectroscopy were collected. Then 33 samples were randomly selected into calibration set and the remaining 11 of the samples were selected into validation set. The authors optimized the pretreatment method, the principal components, the modeling region and the scanning parts when developing the models. The optimized model of discriminant analysis was developed using the pretreatment multiplicative scatter correction (MSC)+Savitzky-Golay filter (SG) smoothing, the region 3 932.14～669.18 cm-1, 4 principal components and the rhizome part. The accuracy of the optimized model got up to 100%. The result demonstrated that infrared microspectroscopy technology combined with DA is of simple operation, rapid, nondestructive and effective, and can be applied to recognize MCG and GCG.
卜海博1，王 峰2，林宏英1，郭志勇1, 袁少雄1，潘丽丽1，许晓嘉1，李向日1*，王钢力2*，林瑞超1, 2 . 显微红外光谱法无损识别林下山参与园参 [J]. 光谱学与光谱分析, 2013, 33(11): 3028-3031.
BU Hai-bo1, WANG Feng2, LIN Hong-ying1, GUO Zhi-yong1, YUAN Shao-xiong1, PAN Li-li1，XU Xiao-jia1, LI Xiang-ri1*, WANG Gang-li2*, LIN Rui-chao1, 2. Nondestructive Recognization of Mountain Cultivated Ginseng and Garden Cultivated Ginseng by FTIR Microspectroscopy . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2013, 33(11): 3028-3031.
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