Abstract：In the present paper, meiosis of male sterile pollen mother cells and fertility of ripened pollen was observed in male sterile line (Xindao97200A) by using laser scanning confocal microscopy (LSCM), beside, the seed setting rate of selfing and artificial pollination was investigated. Abnormal chromosome behaviors during the meiosis of PMC were found with 15.8%, which included lagging chromosome, dragging chromosomes, three poles of spindles, chromosome bridge, micronuclei and asynchrony of the dyad. Typical, spherical and stained abortive rates were 6.15%, 10.26% and 80.77%, respectively. The pollen abortion might be attributed to abnormal pollen development, which resulted from chromosome pairing abnormally, abnormal division, chromosome breakage and unevenly distributed cytoplasm.
张佳佳，代西梅*，赵帅鹏，刘贺梅，黄群策，焦 浈 . 激光扫描共聚焦显微技术对水稻不育系花粉减数分裂的特性研究 [J]. 光谱学与光谱分析, 2013, 33(09): 2483-2487.
ZHANG Jia-jia, DAI Xi-mei*, ZHAO Shuai-peng, LIU He-mei, HUANG Qun-ce, JIAO Zhen . Observation of the Pollen Mother Cell Meiosis of Male Sterile Line in Rice by Using Confocal Microscopy . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2013, 33(09): 2483-2487.
[1] GUO Yu-hua, ZHANG LI-shuang, LIN Tuan-rong, et al(郭玉华, 张立爽, 林团荣, 等). Jiangsu Agricultural Sciense(江苏农业科学), 2010, 1: 91. [2] Sujin, K, Hyejin Y, Hak S S, et al. Planta, 2003, 217: 559. [3] He J H, Muhammad Q S, Chen Z X, et al. Plant Syst. Evol., 2011, 291, 257. [4] ZHAO Ming-hui, LIU Xiang-dong, LU Yong-gen, et al(赵明辉, 刘向东, 卢永根, 等). Journal of Crops(作物学报). 2006, 32(10): 1472. [5] DAI Xi-mei, HUANG Qun-ce, LI Guo-ping, et al(代西梅, 黄群策, 李国平, 等). Chinese Journal of Rice Science(中国水稻科学)，2006, 20(2), 165. [6] ZHANG Jia-jia, DAI Xi-mei, LIU He-mei, et al(张佳佳, 代西梅, 刘贺梅, 等). Journal of Zhengzhou Univerdity(郑州大学学报·自然科学版)，2012, 44(4)：105. [7] YANG Hong-yuan(杨宏远). Rice Reproductive Biology(水稻生殖生物学). Hangzhou: Zhejiang University Press(杭州: 浙江大学出版社), 2005. 24. [8] DAI Xi-mei(代西梅). Zhengzhou: Zhengzhou University(郑州：郑州大学), 2006. [9] He J H, Shahid M Q, Li Y J, et al. Journal of Experimental Botany, 2011, 62: 4433. [10] YANG Xu(杨 旭). Zhengzhou: Zhengzhou University(郑州：郑州大学), 2010. [11] ZHANG Yue, TAO Cai-qin, LONG Zhen, et al(张 悦, 陶才琴, 龙 振, 等). Seed(种子). 2010, 29(7): 5. [12] HUANG Xing-guo, WANG Guang-yong, YU Jin-hong, et al(黄兴国, 汪广勇, 余金洪, 等). Chinese Journal of Rice Science(中国水稻科学). 2011, 25(4), 370.
