Study on the UV-Quantitative Analysis of Theabrownins in Pu-Erh Tea
YANG Yan1, PENG Chun-xiu2*, LIU Hua-rong1*, SHENG Jun1, GONG Jia-shun1
1. Faculty of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, China 2. Faculty of Garden and Horticultural, Yunnan Agricultural University, Kunming 650201, China
Abstract:An UV-quantitative analysis method for the theabrownin (TB) in Pu-erh tea and its derived products was established in the present study. The results showed that the Pu-erh tea shows characteristic absorption at the wavelength of 270 nm, which can be used as wavelength for the content determination of TB. The preparation methods of standard TB and standard curve were also established. The determination results show that the method is simple, the results have certain credibility, and the established method can be used for the determination of TB in Pu-erh tea and its derived products.
杨 妍1,彭春秀2*,刘华戎1*,盛 军1,龚加顺1 . 普洱茶中茶褐素含量的紫外光谱法测定 [J]. 光谱学与光谱分析, 2013, 33(07): 1850-1856.
YANG Yan1, PENG Chun-xiu2*, LIU Hua-rong1*, SHENG Jun1, GONG Jia-shun1 . Study on the UV-Quantitative Analysis of Theabrownins in Pu-Erh Tea . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2013, 33(07): 1850-1856.
[1] Cao Z H, Gu D H, Lin Q Y, et al. Phytother Research, 2011, 25(2): 234. [2] Gong J S,Peng C X, Chen T, et al. Journal of Food Science,2010,75(6):182. [3] GONG Jia-shun, ZHOU Hong-jie(龚加顺,周红杰). Yunnan Pu-Erh Tea Chemistry(云南普洱茶化学). Kunming: Yunnan Science and Technology Press(昆明:云南科技出版社),2011. 10. [4] Gong J S,Chao Tang, Peng C X. Journal of Analytical and Applied Pyrolysis, 2012,95:189. [5] Roberts E H, Smith R. Journal of the Science of Food and Agriculture, 1963,14: 689. [6] Hilton P J, Tea In F D, Snell L C. Ettre(Eds.). Encyclopedia of Industrial Chemical Analysis(Vol. 18, pp. 455–516). New York: John Wiley,1973. [7] WANG Kun-bo, LIU Zhong-hua, HUANG Jian-an(王坤波,刘仲华,黄建安). Food Science(食品科学),2001,22(11):104. [8] Lee B L, Ong C N. Journal of Chromatography A, 2000, 881: 439. [9] Lee M J, Prabhu S, Meng X, et al. Analytical Biochemistry, 2000, 279(2): 164. [10] Whitehead D L, Temple C M. Journal of Science Food and Agriculture, 1992, 58(1): 149. [11] HUANG Yi-huan(黄意欢). Experimental Technology of Tea(茶学实验技术). Beijing: China Agricultural Press(北京: 中国农业出版社),1995. [12] GONG Jia-shun, ZHOU Hong-jie, ZHANG Xin-fu, et al(龚加顺,周红杰,张新富,等). Journal of Tea Science(茶叶科学),2005,25(1):44. [13] LUO Long-xin, WU Xiao-chong, DENG Yu-liang(罗龙新,吴小崇,邓余良). Journal of Tea Science(茶叶科学),1998,18(1):53. [14] LIANG Ming-zhi, XIA Li-fei, CHEN Lin-bo,et al(梁名志, 夏丽飞, 陈林波,等). Chinese Agricultural Science Bulletin(中国农学通报), 2006, 22(10): 321.