Quantitative Analysis of Protein Concentration by Absorption Peak Integration Method of UV Spectroscopy—Taking Alkaline Phosphatase as an Example
ZHU Yuan-rong1, 2, WU Feng-chang1*, LIN Ying3
1. State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China 2. College of Water Sciences, Beijing Normal University, Beijing 100875, China 3. Appraisal Center for Environment & Engineering Ministry of Environmental Protection, Beijing 100012, China
Abstract：Adsorption of alkaline phosphatase (APase) on minerals (goethite, montmorillonite) and sediments from Taihu Lake was studied. However, the concentration of alkaline phosphatase in the supernatant cannot be analyzed by the adsorption at 280 nm due to that the UV spectroscopy was changed. Quantitative analysis of alkaline phosphatase in the supernatant by the absorption peak (252~305 nm) integration method of UV spectroscopy was developed. This method determined the concentrations of alkaline phosphatase well. Compared to the results of Bradford, the absorption peak integration method can determine the concentrations of supernatant fast, conveniently, and accurately. This method can also be applied to other protein solution analysis and similar experiments. The drawbacks of traditional single wavelength method (280nm) were overcome to some extent based on the method of absorption peak integration.
朱元荣1, 2，吴丰昌1*，林 樱3 . 紫外吸收光谱积分法分析蛋白质浓度-以碱性磷酸酶为例 [J]. 光谱学与光谱分析, 2013, 33(07): 1845-1849.
ZHU Yuan-rong1, 2, WU Feng-chang1*, LIN Ying3 . Quantitative Analysis of Protein Concentration by Absorption Peak Integration Method of UV Spectroscopy—Taking Alkaline Phosphatase as an Example . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2013, 33(07): 1845-1849.
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