Application of Visible and Near Infrared Spectroscopy for Rapid and Non-Invasive Quantification of Adulterants in Fish Oil
ZHANG Yu1,2, TAN Li-hong1, CAO Fang2, HE Yong2*
1. Zhejiang Technical Institute of Economics, Hangzhou 310018, China 2. College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China
Abstract：Visible and near infrared (Vis-NIR) spectroscopy was used to accomplish a rapid and noninvasive quantification of the two common adulterants, soybean oil and rapeseed oil, in fish oil. Different contents of soybean oil were added into fish oil of three brands and different contents of rapeseed oil were added into fish oil of another three brands, the Vis-NIR spectra of adulterated samples were collected, pretreated by five spectral preprocessing algorithms (smoothing, standard normal variate (SNV), multiplicative scatter correction (MSC), 1st-derivative, and 2nd- derivative), and used to establish partial least square regression (PLSR) models. The correlation coefficients for prediction (Rp) of 0.938 6 and 0.959 3 were obtained for the adulterant detection of soybean oil and rapeseed oil respectively, and their optimal models were full range spectral PLSR model and MSC-PLSR model. Successive projections algorithm (SPA) was then used to analyze the full range spectra of fish oil samples adulterated with soybean oil and rapeseed oil respectively, and 11 and 15 spectral characteristic wavelength variables were obtained. The Rp of 0.941 2 and 0.932 6 were obtained based on SPA-PLSR models for the adulterant detection of soybean oil and rapeseed oil, respectively. The overall results indicate that Vis-NIR spectroscopy is a feasible way to determine the adulterants of soybean oil and rapeseed oil in fish oil rapidly and non-destructively.
Key words：Fish oil;Adulterants;Visible and near infrared spectroscopy;Successive projections algorithm(SPA)
张 瑜1,2，谈黎虹1，曹 芳2，何 勇2* . 可见-近红外光谱分析技术对鱼油掺假定量快速无损检测方法研究 [J]. 光谱学与光谱分析, 2013, 33(06): 1532-1536.
ZHANG Yu1,2, TAN Li-hong1, CAO Fang2, HE Yong2* . Application of Visible and Near Infrared Spectroscopy for Rapid and Non-Invasive Quantification of Adulterants in Fish Oil. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2013, 33(06): 1532-1536.
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