Raman Spectra of Different Kinds of Thalassemia Erythrocytes with the Effect of pH
WU Zheng-jie1, WANG Cheng2, LIN Zheng-chun3
1. Department of Chemical Engineering and Materials Science, Beijing Institute of Technology, Zhuhai, Zhuhai 519088, China 2. Department of Computer Science and Engineering, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China 3. Foshan Institute of Technological Standardization, Foshan 528000, China
Abstract：Thalassemia is a kind of blood diseases which has high morbidity and large influence. Previous methods for diagnosis are all very cumbersome and time consuming. By comparing Raman spectra of different kinds of thalassemia and normal erythrocytes at acid or alkaline pH, it was found that β-thalassemia and α-thalassemia erythrocytes have dissimilar Raman spectra in the acidic environment, such as the Raman spectra of β-thalassemia erythrocytes showed higher intensity at the characteristic bands assigned to oxyhemoglobin, and the characteristic bands assigned to deoxyhemoglobin were even completely replaced; β-thalassemia erythrocytes membrane has a smaller chain interaction between the transverse order parameters than normal erythrocytes, and the Slat values are different for different stages of anemia, while the Slat values are similar between α-thalassemia and normal erythrocytes, indicating that based on the effect of pH it is possible to diagnose thalassemia more quickly by using Raman spectra.
吴正洁1，王 成2，林正春3 . pH值对多类人地贫红细胞作用的拉曼光谱研究 [J]. 光谱学与光谱分析, 2013, 33(04): 982-986.
WU Zheng-jie1, WANG Cheng2, LIN Zheng-chun3 . Raman Spectra of Different Kinds of Thalassemia Erythrocytes with the Effect of pH . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2013, 33(04): 982-986.
[1] Advani R, Sorenson S, Shinar E, et al. Blood, 1992, 79(4): 1058. [2] Mannu F, Arese P, Cappellini M D, et al. Blood, 1995, 86(5): 2014. [3] Yuan J, Bunyaratvej A, Fucharoen S, et al. Blood, 1995, 86(10): 3945. [4] Kuypers F A, Yuan J, Lewis R A, et al. Blood, 1998, 91(8): 3044. [5] Eldor A, Rachmilewitz E A. Blood, 2002, 99(1): 36. [6] LI Chang-gang, SHI Hong-song, WANG Ying, et al(李长钢, 石红松, 王 缨, 等). Journal of Chinese Morden Pediatrics(中华现代儿科学杂志), 2004, 1(3): 210. [7] PAN Gan-hua(潘干华). Chinese Journal of Birth Health and Heredity(中国优生与遗传杂志), 2007, 15(7): 14. [8] KANG Li-li, HUANG Yao-xiong, WU Zheng-jie(康立丽, 黄耀熊, 吴正洁). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2011, 31(2): 408. [9] YU Zhi-fei(郁知非). Modern hematology(现代血液病学). Hangzhou: Zhejiang Science Press(杭州: 浙江科技出版社), 1989. [10] Tu A T. Raman Spectroscopy in Biology: Principles and Applications. New York: Wiley, 1982. [11] XU Yi-ming(许以明). Raman Spectroscopy and Its Application in Structural Biology(拉曼光谱及其在结构生物学中的应用). Beijing: Chemical Industry Press(北京: 化学工业出版社), 2005. [12] Kuypers F A, Yuan J, Lewis R A, et al. Blood, 1998, 91(8): 3044. [13] Brunori M, Falcioni G, Fioretti E, et al. European Journal of Biochemistry, 1975, 53: 99. [14] Bank A. Journal of Clinical Investigation, 2007, 117: 1746. [15] Abe M, Kitagawa T, Kyogoku Y. Journal of Chemical Physics, 1978, 69(10): 4526. [16] Rousseau D L, Song S, Friedman J M, et al. Journal of Biological Chemistry, 1992, 268(8): 5719.
