Abstract：In order to observe the neurotrophin’s influence on intracellular free calcium concentration, the test neuron was labeled by fluorescence indicator Fluo-3, and imaged by self-built real-time fluorescence microscopy system. The authors observed the changes in intracellular free calcium concentration ([Ca2+]i) in the bovine retinal neurons under the influence of four kinds of neurotrophins such as brain derived neurotrophic factor BDNF. On account of the fact that fluorescence indicator’s intensity decays over time, it is necessary to apply a “decay removal correction” to the fluorescence intensity in order to show the fluorescence intensity that solely represents [Ca2+]i. The corrected data shows an increase after adding neurotrophins to neurons, which is consistent with similar results published by other groups. Thus, our method of imaging living cells is feasible, and “decay removal correction” is reliable.
杨 寻，刘晓晨，马万云* . 单个牛视网膜神经细胞内游离Ca2+动态变化的实时观测方法 [J]. 光谱学与光谱分析, 2013, 33(04): 877-880.
YANG Xun, LIU Xiao-chen, MA Wan-yun* . A Method for Real-Time Observation of Intracellular Free Ca2+ Concentration in Single Bovine Retinal Neurons. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2013, 33(04): 877-880.
[1] Jeff W Barclay, Alan Morgan, Robert D Burgoyne. Cell Calcium, 2005, 38: 343. [2] Robert D Burgoyne，Lee P Haynes. Molecular Brain, 2012, 5: 2. [3] Augustine G J, Santamaria F, Tanaka K. Neuron, 2003, 40: 331. [4] Michelle D Amaral，Lucas Pozzo-Miller. Journal of Neurophysiology, 2007, 98: 2476. [5] Quintanar-Escorzab M A, González-Martínezc M T, Intriago-Ortega Ma del Pilar. Toxicology in Vitro, 2010, 24(5): 1338. [6] Wang Xiaojing, Zheng Honghua, Liu Changjin, et al. Neurochemical Research, 2008, 33: 826. [7] Robin J Kleiman, Ning Tian, David Krizaj. Journal of Neurophysiology, 2000, 84: 1472. [8] Michelle D Amaral，Lucas Pozzo-Miller. Neural Plasticity，2012; 2012: 203536. [9] http://products.invitrogen.com/ivgn/product/F23915.
