Baseline Correction of Raman Spectrum Based on Piecewise Linear Fitting
QIN Zhao-jun1, 2, TAO Zhan-hua2, LIU Jun-xian1*, WANG Gui-wen2*
1. College of Physics Sciences & Technology, Guangxi Normal University, Guilin 541004, China 2. Laboratory of Biophysics, Guangxi Academy of Sciences, Nanning 530007, China
Abstract：The baseline drifts of Raman spectra occur in many types of instrumental measurements. It is an important part and a routine step to correct the baseline drift for the data preprocessing. In the present work, the limitations of the baseline correction method based on polynomial fitting were highlighted and a modified polynomial fitting method, i.e. piecewise linear fitting method, was proposed. Combined with the computer, this method could eliminate the baseline automatically. A series of Raman spectra of single polystyrene bead, red blood cell or yeast cell acquired by laser tweezers Raman spectroscopy were preprocessed by this method and its efficiency was verified. The results demonstrated that piecewise linear fitting can correct the baseline shifts effectively and provides more accurate information for further data analysis. It is a feasible method for correction of Raman spectrum.
覃赵军1, 2，陶站华2，刘军贤1*，王桂文2* . 分段式线性拟合校正拉曼光谱基线漂移[J]. 光谱学与光谱分析, 2013, 33(02): 383-386.
QIN Zhao-jun1, 2, TAO Zhan-hua2, LIU Jun-xian1*, WANG Gui-wen2* . Baseline Correction of Raman Spectrum Based on Piecewise Linear Fitting . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2013, 33(02): 383-386.
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