Research on Polarized Three-Dimensional Fluorescence Spectra of Milk Solution
LAN Xiu-feng1, GU Chun-feng1, YU Yin-shan1, LU Li-ping2
1. College of Science, Nanjing University of Aeronautics and Astronautics, Nanjing 210016, China 2. College of Science, Nanjing Agricultural University, Nanjing 210095, China
Abstract：Polarized three-dimensional fluorescence spectra of different kinds of milk were measured, including pure milk, milk with high calcium, and milk with high calcium but low fat produced by YiLi and MengNiu. It was found that the fluorescence intensity is stronger when excitation and emission polarizer orientation is parallel. The angle between excitation dipole and emission dipole of all kinds of milk equivalent chromophore was estimated by a simple model, indicating that they all less than 40°. The angle for milk with high calcium but low fat is less than that of pure milk and milk with high calcium. And in the experiment, the polarized fluorescence intensity of milk with high calcium but low fat is significantly greater than the others, which fitted with the model estimation.
[1] XU Yun, WANG Yi-ming, WU Jing-zhu, et al(徐 云，王一鸣，吴静珠，等). Journal of Infrared and Millimeter Waves(红外与毫米波学报), 2010, 29(1): 53. [2] Lü Jian-bo(吕建波). Modern Electronics Technique(现代电子技术), 2011, 34(17): 166. [3] RUAN Gong-cheng, LI Wei, XU Fei, et al(阮功成，李 伟，徐 斐，等). Science and Technology of Food Industry(食品工业科技), 2011, 37(7): 176. [4] YANG Rong-bing, LI Chun-yu(杨荣兵，李春雨). China Practical Medicine(中国实用医药), 2011，(33): 249. [5] WANG Shuo, XU Ke-xin(王 硕，徐可欣). Infrared(红外), 2006, 27(11): 27. [6] GU Chun-feng, LAN Xiu-feng, YU Yin-shan, et al(顾春峰，兰秀风，于银山，等). Acta Photonica Sinica(光子学报), 2012, 41(1): 107. [7] XU Jin-gou, WANG Zun-ben(许金钩，王尊本). Fluorescence Analysis(荧光分析法). Beijing: Science Press(北京: 科学出版社), 2006. 15. [8] Yengo C M, Berger C L. Current Opinion in Pharmacology, 2010, 10(6): 731. [9] Hon P, Chu P, Cheng C H, et al. Journal of Chromatography A, 2011, 1218(39): 6907. [10] Men Z W, Fang W H, Ding Y F, et al. Journal of Raman Spectroscopy, 2009, 40(8): 1039. [11] HAN Cai-qin, LIU Ying, ZHAO Wen-yan(韩彩芹，刘 莹，赵文艳，等). Acta Optica Sinica(光学学报), 2009, 29(11): 3163. [12] LIU Jing-wang, ZHANG Bo-yang, LI Shu-feng, et al(刘景旺，张博洋，李树峰，等). The Journal of Light Scattering(光散射学报), 2010, 22(3): 291. [13] WANG Xue-yan, LIU Xiao-wei, CHAO Ruo-bing(王雪彦，刘小玮，晁若冰). Chinese Journal of Pharmaceutical Analysis(药物分析杂志), 2011, 31(8): 1545. [14] YAO Qi-jun(姚启钧). Optical Tutorial(光学教程). Beijing: Higher Education Press(北京：高等教育出版社), 2002. 309.
